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AB302552

Alexa Fluor® 647 Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118]

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(1 Publication)

Rabbit Recombinant Monoclonal NCAP antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Transfected cell lysate - SARS-CoV-2 samples. Cited in 1 publication.

View Alternative Names

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

4 Images
Flow Cytometry - Alexa Fluor® 647 Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (AB302552)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (AB302552)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) cells transfected with SARS-CoV-2 nucleocapsid protein expression vector containing a myc-his tag cells labelling SARS-CoV-2 nucleocapsid protein with ab302552 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal isotype control - Alexa Fluor® 647 / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Immunocytochemistry - Alexa Fluor® 647 Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (AB302552)
  • ICC

Supplier Data

Immunocytochemistry - Alexa Fluor® 647 Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (AB302552)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK293T (human embryonic kidney epithelial cell) cells labelling SARS-CoV-2 nucleocapsid protein with ab302552 at 1/50 (10.0 ug/ml) dilution, followed by antibody at 1/None dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged SARS-CoV-2 Nucleocapsid protein expression vector. is observed. ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is at 1/None dilution.

Flow Cytometry - Alexa Fluor® 647 Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (AB302552)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (AB302552)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) cells transfected with SARS-CoV-1 nucleocapsid protein expression vector containing a myc-his tag (Left) / HEK-293T (human embryonic kidney) cells transfected with MERS nucleocapsid protein expression vector containing a myc-his tag (Right) cells labelling SARS-CoV-2 nucleocapsid protein with ab302552 at 1/500 dilution (0.1ug)/ Right (Red) compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Immunocytochemistry - Alexa Fluor® 647 Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (AB302552)
  • ICC

Supplier Data

Immunocytochemistry - Alexa Fluor® 647 Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (AB302552)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK293T (human embryonic kidney epithelial cell) cells labelling SARS-CoV-2 nucleocapsid protein with ab302552 at 1/50 (10.0 ug/ml) dilution, followed by antibody at 1/None dilution (Green). Confocal image showing no staining in 293T cells transfected with myc-tagged SARS-CoV-1 and MERS Nucleocapsid protein, which are negative controls for SARS-CoV-2. is observed. ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is at 1/None dilution.

  • 578 PE

    PE Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118]

  • Unconjugated

    Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein

  • Carrier free

    Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24334-118

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

SARS-CoV-2

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - SARS-CoV-2": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.
Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed : 33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed : 34901782, PubMed : 36534661).. May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed : 35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed : 35921414).. May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.
See full target information N
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature microbiology 8:1587-1599 PubMed37443277

2023

LY6E is a pan-coronavirus restriction factor in the respiratory tract.

Applications

Unspecified application

Species

Unspecified reactive species

Katrina B Mar,Alexandra I Wells,Marley C Caballero Van Dyke,Alexandra H Lopez,Jennifer L Eitson,Wenchun Fan,Natasha W Hanners,Bret M Evers,John M Shelton,John W Schoggins
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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