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AB300675

Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9]

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Rabbit Recombinant Monoclonal SARS CoV-2 ORF3a Protein antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2 samples.

View Alternative Names

ORF3a protein, ORF3a, Accessory protein 3a, Protein 3a, Protein U274, Protein X1, 3a

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (AB300675)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (AB300675)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with Myc tagged SARS-CoV-2 Protein 3a construct cells labelling SARS CoV-2 ORF3a Protein with ab300675 at 1/100 (5.0 ug/ml) msd2577,(Green). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged SARS-CoV-2 Protein 3a expression vector, and no staining in 293T cells transfected with myc-tagged SARS-COV-1 Protein 3a expression vector. is observed. Myc-Tag Mouse mAb (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 0.5ug/ml msd2577 (Red). The Nuclear counterstain was DAPI (Blue).

  • Unconjugated

    Anti-SARS-CoV2 orf3a antibody [EPR24848-9]

  • Carrier free

    Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24848-9

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

SARS-CoV-2

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - SARS-CoV-2": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 ORF3a protein also known as ORF3 is an accessory protein of the SARS-CoV-2 virus weighing approximately 275 amino acids. It is expressed in infected host cells and plays an important role in the virus's lifecycle. ORF3a is known for its role as an ion channel involved in the viral replication cycle. Its functions include altering host cell processes to facilitate viral propagation and possibly contributing to viral pathogenesis.
Biological function summary

The SARS-CoV-2 ORF3a protein interferes with the host immune response promoting virus survival and replication. ORF3a is implicated in the enhancement of viral pathogenic effects by modulating innate and adaptive immunity. Though not part of a large known stable complex ORF3a interacts with other viral and host proteins impacting processes like apoptosis and inflammation.

Pathways

ORF3a affects several critical processes within the host cell. It plays a role in the apoptosis pathway influencing cell death mechanisms that help the virus evade immune defenses. Additionally it modulates the NF-kB signaling pathway which is vital for immune response regulation. Other related proteins include ORF7a and NSP6 which work alongside ORF3a to manipulate host cell environments to the virus's advantage.

The SARS-CoV-2 ORF3a protein is directly connected to COVID-19 impacting the severity and progression of the disease. Its interaction with host proteins contributes to dysregulated immune responses and cytokine storms characteristic of severe COVID-19 cases. The protein's role in apoptosis links it to potential lung tissue damage exacerbating respiratory conditions associated with COVID-19 infections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in viral egress via lysosomal trafficking (PubMed : 33157038, PubMed : 33422265). Forms homotetrameric ion channels (viroporins) localized at endosomes and lysosomes, that may induce deacidification of lysosomes, allowing safe egress of virions via lysosomal trafficking (PubMed : 33157038, PubMed : 33422265, PubMed : 34158638). Also blocks autolysosome formation by binding and sequestering the host component VPS39 for homotypic fusion and protein sorting (HOPS) on late endosomes (PubMed : 33422265). This prevents fusion of autophagosomes with lysosomes, disrupting autophagy and facilitating virus egress (PubMed : 33422265). Induces host RETREG1/FAM134B-dependent reticulophagy by interacting with host HMGB1 and enhancing the association between HMGB1 and host BECN1 (PubMed : 35239449). This induces endoplasmic reticulum stress and inflammatory responses and facilitates viral infection (PubMed : 35239449).
See full target information 3a
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com