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AB196536

Alexa Fluor® 647 Anti-SATB2 antibody [EPNCIR130A]

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(4 Publications)

Rabbit Recombinant Monoclonal SATB2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

KIAA1034, SATB2, DNA-binding protein SATB2, Special AT-rich sequence-binding protein 2

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SATB2 antibody [EPNCIR130A] (AB196536)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SATB2 antibody [EPNCIR130A] (AB196536)

ab196536 staining SATB2 in HT1080 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196536 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-SATB2 antibody [EPNCIR130A] (AB196536)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-SATB2 antibody [EPNCIR130A] (AB196536)

Overlay histogram showing A431 cells stained with ab196536 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab196536, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 25mW Red Solid State Diode laser (635nm) and 675/30 bandpass filter.

This antibody gave a positive signal in A431 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A]

  • 578 PE

    PE Anti-SATB2 antibody [EPNCIR130A]

  • 660 APC

    APC Anti-SATB2 antibody [EPNCIR130A]

  • Unconjugated

    Anti-SATB2 antibody [EPNCIR130A]

  • Carrier free

    Anti-SATB2 antibody [EPNCIR130A] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR130A

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p>This product gave a positive signal in HT1080 cells fixed with 4% formaldehyde (10 min)</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SATB2 protein also known as Special AT-rich Sequence-Binding Protein 2 is a DNA-binding protein with a mass of about 82 kDa. It plays a role in chromatin organization and transcriptional regulation by binding to AT-rich sequences within the nuclear matrix. SATB2 shows high expression levels in the cerebral cortex and the bone where it influences gene expression. It functions by facilitating the assembly of transcriptional machineries enhancing or repressing the transcription of its target genes.
Biological function summary

SATB2 acts as a regulator of multiple developmental processes. It serves as a master control gene in osteoblast differentiation and skeletal development by forming complexes with core binding factors. In the brain SATB2 contributes to the development and maintenance of neuronal networks that are essential for cognitive functions. Its binding to matrix attachment regions aids in the spatial organization of nuclear chromatin impacting gene expression pertinent to development and differentiation.

Pathways

SATB2 operates within the Wnt signaling pathway and the regulation of osteoblast differentiation. It interacts with proteins such as RUNX2 and β-catenin which play roles in bone formation and homeostasis. Within these pathways SATB2 modulates gene networks that influence the expression of structural and signaling proteins important for tissue differentiation and repair particularly in bone and neuronal contexts.

SATB2 has associations with cleft palate and intellectual disabilities conditions often related to alterations in craniofacial and neurological development. It interacts with proteins like SOX9 and TCF7L1 which have roles in developmental processes that when disrupted can lead to such conditions. Understanding SATB2's involvement in these disorders provides insights into potential therapeutic targets for interventions aiming to mitigate developmental abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
See full target information SATB2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Science (New York, N.Y.) 382:eadf6812 PubMed37824655

2023

Transcriptomic cytoarchitecture reveals principles of human neocortex organization.

Applications

Unspecified application

Species

Unspecified reactive species

Nikolas L Jorstad,Jennie Close,Nelson Johansen,Anna Marie Yanny,Eliza R Barkan,Kyle J Travaglini,Darren Bertagnolli,Jazmin Campos,Tamara Casper,Kirsten Crichton,Nick Dee,Song-Lin Ding,Emily Gelfand,Jeff Goldy,Daniel Hirschstein,Katelyn Kiick,Matthew Kroll,Michael Kunst,Kanan Lathia,Brian Long,Naomi Martin,Delissa McMillen,Trangthanh Pham,Christine Rimorin,Augustin Ruiz,Nadiya Shapovalova,Soraya Shehata,Kimberly Siletti,Saroja Somasundaram,Josef Sulc,Michael Tieu,Amy Torkelson,Herman Tung,Edward M Callaway,Patrick R Hof,C Dirk Keene,Boaz P Levi,Sten Linnarsson,Partha P Mitra,Kimberly Smith,Rebecca D Hodge,Trygve E Bakken,Ed S Lein

Molecules and cells 45:588-602 PubMed35754370

2022

RNA Binding Protein Rbms1 Enables Neuronal Differentiation and Radial Migration during Neocortical Development by Binding and Stabilizing the RNA Message for Efr3a.

Applications

Unspecified application

Species

Unspecified reactive species

Khadija Habib,Kausik Bishayee,Jieun Kang,Ali Sadra,Sung-Oh Huh

International journal of nanomedicine 15:10539-10546 PubMed33408473

2020

Development of a Novel Homogeneous Nanoparticle-Based Assay for Rapid and High-Throughput Quantitation of the sST2 Protein in Human Serum.

Applications

Unspecified application

Species

Unspecified reactive species

Shenxia Gao,Junpu Li

Nature 587:644-649 PubMed33057195

2020

Cell-type-specific 3D epigenomes in the developing human cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Song,Mark-Phillip Pebworth,Xiaoyu Yang,Armen Abnousi,Changxu Fan,Jia Wen,Jonathan D Rosen,Mayank N K Choudhary,Xiekui Cui,Ian R Jones,Seth Bergenholtz,Ugomma C Eze,Ivan Juric,Bingkun Li,Lenka Maliskova,Jerry Lee,Weifang Liu,Alex A Pollen,Yun Li,Ting Wang,Ming Hu,Arnold R Kriegstein,Yin Shen
View all publications
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Product promise

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