JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB222919

Alexa Fluor® 647 Anti-SC35 antibody [EPR12238]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal SC35 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

SFRS2, SRSF2, Serine/arginine-rich splicing factor 2, Protein PR264, Splicing factor SC35, SC-35

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SC35 antibody [EPR12238] (AB222919)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SC35 antibody [EPR12238] (AB222919)

ab222919 staining SC35 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab222919 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12238

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)</p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SC35 also known as SRSF2 (serine/arginine-rich splicing factor 2) is an essential splicing factor with a molecular mass around 26 kDa. This protein is part of the SR protein family which are known for their roles in splicing regulation due to their serine/arginine-rich domains. SC35 predominantly localizes in the nucleus of cells where it accumulates in nuclear speckles areas rich in splicing factors. Biologists often use immunofluorescence techniques and anti-SC antibodies to visualize and study SC35's localization and function within cells.
Biological function summary

SC35 influences various aspects of mRNA processing particularly pre-mRNA splicing. The protein functions as a splicing enhancer by interacting with pre-mRNA at specific sites to assist exon inclusion during splicing. SC35 acts as part of the spliceosomal complex which is critical during the assembly and rearrangement of the spliceosome. Additionally it interacts with other splicing factors to ensure accurate and efficient splicing which is fundamental for gene expression and consequent protein synthesis.

Pathways

The SC35 protein participates in the RNA splicing pathway a significant process in mRNA maturation and gene expression regulation. This protein interacts with other SR proteins like SRSF1 and components of the spliceosomal machinery. SC35's function is also important in the alternative splicing pathway impacting the diversity of mRNA transcripts through the production of different exon combinations. By regulating alternative splicing SC35 helps produce protein diversity vital for numerous cellular processes.

Changes in SC35 expression or function have been linked to several human conditions. Notably its dysregulation can contribute to cancer development through altered splicing patterns that impact oncogene and tumor suppressor gene expression. Furthermore SC35 abnormalities are associated with spinal muscular atrophy a neurodegenerative disorder where splicing regulation plays a significant role. In these diseases SC35 may interact with other proteins such as SMN (survival of motor neuron protein) and impact their functions highlighting its relevance in maintaining cellular health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.
See full target information SRSF2
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB229473

Anti-SC35 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SC35 antibody - N-terminal (AB229473)

  • 0
  • 0
Primary Antibodies

AB264431

Anti-SC35 antibody [SC-35] - BSA and Azide free

Immunocytochemistry - Anti-SC35 antibody [SC-35] - BSA and Azide free (AB264431)

  • 0
  • 0
Primary Antibodies

AB11826

Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

  • 4
  • 16
Primary Antibodies

AB28428

Anti-SC35 antibody

BOND RX™ Validated

Western blot - Anti-SC35 antibody (AB28428)

  • 0
  • 2

Complementary Products

Primary Antibodies

AB166630

Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker (AB166630)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB210785

Anti-Coilin antibody

KO Validated

Western blot - Anti-Coilin antibody (AB210785)

  • 0
  • 0
Primary Antibodies

AB29

Anti-PCNA antibody [PC10]

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PCNA antibody [PC10] (AB29)

  • 5
  • 72

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com