JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB288363

Alexa Fluor® 647 Anti-Scn1a antibody [EPR22326-112]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal SCN1A antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr, IHC-P and reacts with Mouse samples.

View Alternative Names

Sodium channel protein type 1 subunit alpha, Sodium channel protein brain I subunit alpha, Sodium channel protein type I subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.1, Scn1a

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Scn1a antibody [EPR22326-112] (AB288363)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Scn1a antibody [EPR22326-112] (AB288363)

Immunofluorescent staining of Scn1a in a section of formalin-fixed paraffin-embedded mouse normal brain.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab288363 at 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Scn1a antibody [EPR22326-112] (AB288363)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Scn1a antibody [EPR22326-112] (AB288363)

Negative immunofluorescence staining of Scn1a in a section of frozen mouse normal heart.

The section was fixed using 10% formaldehyde in PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND®. The section was incubated at room temperature for 1 hour with ab288363 at 1/50 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Scn1a antibody [EPR22326-112] (AB288363)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Scn1a antibody [EPR22326-112] (AB288363)

Negative immunofluorescence staining of Scn1a in a section of formalin-fixed paraffin-embedded mouse normal heart.

Performed on a Leica BOND®. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab288363 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Scn1a antibody [EPR22326-112] (AB288363)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Scn1a antibody [EPR22326-112] (AB288363)

Immunofluorescence staining of Scn1a in a section of frozen mouse normal brain.

The section was fixed using 10% formaldehyde in PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND®. The section was incubated at room temperature for 1 hour with ab288363 at 1/50 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Scn1a antibody [EPR22326-112] (AB288363)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Scn1a antibody [EPR22326-112] (AB288363)

Immunofluorescent staining of Scn1a in a section of frozen mouse normal brain.

Performed on a Leica BOND. The section was incubated at room temperature for 1 hour with ab288363 at 1/50 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22326-112

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Scn1a gene encodes the Nav1.1 protein a voltage-gated sodium channel alpha subunit. This protein plays an important role in the initiation and propagation of action potentials in neurons. Nav1.1 has an approximate mass of 260 kDa and is primarily expressed in the central nervous system especially in interneurons. These channels are pivotal for maintaining normal neuronal excitability and their regulation underpins nerve impulse transmission.
Biological function summary

Nav1.1 function influences the balance of excitatory and inhibitory signals in the brain. It forms part of the sodium channel complex working alongside beta subunits to modulate gating properties and channel stability. Dysfunction in Nav1.1 affects neuronal excitability leading to altered synaptic transmission which can impact various neural processes and result in neurophysiological abnormalities.

Pathways

Nav1.1 has an integral role in excitatory signal pathways and is key to the propagation of action potentials in neurons. It interacts closely with proteins like Nav1.2 and Nav1.6 which are also sodium channels contributing to electric signal transmission in the nervous system. Nav1.1 participates in neuronal communication pathways which impact processes like synaptic plasticity and overall brain function.

Alterations in Nav1.1 activity are strongly associated with epilepsy specifically Dravet syndrome. Mutations in SCN1A can lead to defective sodium channel function increasing susceptibility to seizures. Nav1.1 is also linked to other proteins like GABA receptors as impaired inhibitory signaling is involved in the pathology of epilepsy. Furthermore potential associations exist with migraine disorders given the protein's influence on neuronal excitability and signal transmission.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Pore-forming subunit of Nav1.1, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na(+) ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed : 16921370, PubMed : 17928448, PubMed : 27281198). By regulating the excitability of neurons, ensures that they respond appropriately to synaptic inputs, maintaining the balance between excitation and inhibition in brain neural circuits (PubMed : 16921370, PubMed : 22914087). Nav1.1 plays a role in controlling the excitability and action potential propagation from somatosensory neurons, thereby contributing to the sensory perception of mechanically-induced pain (PubMed : 27281198).
See full target information Scn1a
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB51253

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]

BOND RX™ Validated|RabMAb|Recombinant

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

  • 5
  • 9
Primary Antibodies

AB302827

Alexa Fluor® 488 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB302827)

  • 4
  • 1
Primary Antibodies

AB305115

Alexa Fluor® 488 Anti-CGRP-I+CGRP-II antibody [EPR23804-95]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (AB305115)

  • 0
  • 0
Primary Antibodies

AB302570

Alexa Fluor® 555 Anti-Tau (phospho S396) antibody [EPR2731]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Alexa Fluor® 555 Anti-Tau (phospho S396) antibody [EPR2731] (AB302570)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com