JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB197722

Alexa Fluor® 647 Anti-SDHB antibody [21A11AE7]

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Monoclonal SDHB antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Iron-sulfur subunit of complex II, Malate dehydrogenase [quinone] iron-sulfur subunit, IP, SDHB

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SDHB antibody [21A11AE7] (AB197722)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SDHB antibody [21A11AE7] (AB197722)

ab197722 staining SDHB in wild-type HEK293 cells (top panel) and SDHB knockout HEK293 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab197722 at 1/500 dilution (shown in red) and ab195887 (Mouse monoclonal to alpha Tubulin - Alexa Fluor® 488) at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SDHB antibody [21A11AE7] (AB197722)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SDHB antibody [21A11AE7] (AB197722)

ab197722 staining SDHB in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab197722 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

21A11AE7

Isotype

IgG2a

Light chain type

kappa

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB151684

Anti-SDHB antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody (AB151684)

  • 0
  • 0
Primary Antibodies

AB198329

HRP Anti-SDHB antibody [EPR10880]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-SDHB antibody [EPR10880] (AB198329)

  • 0
  • 0
Primary Antibodies

AB198330

Biotin Anti-SDHB antibody [EPR10880]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-SDHB antibody [EPR10880] (AB198330)

  • 0
  • 0
Primary Antibodies

AB175225

Anti-SDHB antibody [EPR10880]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] (AB175225)

  • 0
  • 0
Primary Antibodies

AB178423

Anti-SDHB antibody [EPR13042(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (AB178423)

  • 5
  • 1

Complementary Products

Assay Kits

AB228560

Succinate Dehydrogenase Activity Assay Kit (Colorimetric)

Functional Studies - Succinate Dehydrogenase Activity Assay Kit (Colorimetric) (AB228560)

  • 0
  • 0
Primary Antibodies

AB154473

Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473)

  • 0
  • 0
Primary Antibodies

AB154477

Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (AB154477)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB14748

Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (AB14748)

  • 5
  • 27
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein SDHB also known as Succinate Dehydrogenase Complex Iron Sulfur Subunit B plays a mechanical role as a component of the succinate dehydrogenase (SDH) enzyme. This subunit has a molecular mass of approximately 30 kDa. SDHB is part of the mitochondrial inner membrane where it is expressed in high levels across various tissues including heart kidney and muscle. It contributes to the electron transport chain by facilitating the oxidation of succinate to fumarate an important step in energy production through the tricarboxylic acid (TCA) cycle.
Biological function summary

SDHB participates in the succinate dehydrogenase complex acting within both the TCA cycle and the electron transport chain. By transferring electrons to coenzyme Q it helps generate an electrochemical gradient important for ATP synthesis. This complex also called Complex II includes other subunits such as SDHA SDHC and SDHD. Their interactions ensure proper function of metabolic processes within mitochondria bridging the gap between foundational energy metabolism and complex cellular processes.

Pathways

SDHB plays an important role in cellular respiration and metabolic cycles. It resides in the TCA cycle and the mitochondrial electron transport chain connecting its function to energy generation pathways. Specifically SDHB relates closely to SDHA and coenzyme Q all working together to facilitate electron transfer and effective mitochondrial energy output. The transfer of electrons through this pathway highlights its essential contribution to maintaining cellular energy homeostasis.

SDHB mutations are associated with conditions such as paraganglioma and pheochromocytoma. These genetic alterations disrupt normal complex II function leading to the accumulation of succinate and succinate-related oncogenesis. In addition to causing tumor development defective SDHB is linked with familial paraganglioma syndromes. Its interaction with proteins such as SDHA and SDHD critical for the succinate dehydrogenase functionality highlights its significant role in maintaining metabolic balance and when defective contributing to disease pathogenesis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed : 2902878). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (PubMed : 1864383, PubMed : 2902878). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (PubMed : 2902878).
See full target information SDHB
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 151: PubMed39431301

2024

The GTPase RAB6 is required for stem cell maintenance and cell migration in the gut epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre Simonin,Gehenna Lobo Guerrero,Sabine Bardin,Ram Venkata Gannavarapu,Denis Krndija,Joseph Boyd,Stephanie Miserey,Danijela Matic Vignjevic,Bruno Goud

Nature immunology 24:991-1006 PubMed37095377

2023

Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yavuz F Yazicioglu,Eros Marin,Ciaran Sandhu,Silvia Galiani,Iwan G A Raza,Mohammad Ali,Barbara Kronsteiner,Ewoud B Compeer,Moustafa Attar,Susanna J Dunachie,Michael L Dustin,Alexander J Clarke
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com