JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB270169

Alexa Fluor® 647 Anti-Siglec 7 antibody [EPR23118-130]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Siglec 7 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD328, AIRM1, SIGLEC7, Sialic acid-binding Ig-like lectin 7, Siglec-7, Adhesion inhibitory receptor molecule 1, CDw328, D-siglec, QA79 membrane protein, p75, AIRM-1

1 Images
Flow Cytometry - Alexa Fluor® 647 Anti-Siglec 7 antibody [EPR23118-130] (AB270169)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 647 Anti-Siglec 7 antibody [EPR23118-130] (AB270169)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab270169 (right) or Rabbit IgG (monoclonal) Alexa Fluor® 647 isotype (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10μg/mL human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab270169) or Rabbit IgG (monoclonal) Alexa Fluor® 647 isotype (100 μl at 0.2μg/mL (1/2500)) for 30 min on ice. The cells were simultaneously stained with NCAM.

Acquisition of >30000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter. Events were gated on Live lymphocytes.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Siglec 7 antibody [EPR23118-130]

  • 578 PE

    PE Anti-Siglec 7 antibody [EPR23118-130]

  • Unconjugated

    Anti-Siglec 7 antibody [EPR23118-130]

  • Carrier free

    Anti-Siglec 7 antibody [EPR23118-130] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23118-130

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/2500", "FlowCyt-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).
See full target information SIGLEC7
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB267615

Human Siglec 7 ELISA Kit

Sandwich ELISA - Human Siglec 7 ELISA Kit (AB267615)

  • 0
  • 0
Primary Antibodies

AB234263

PerCP/Cy5.5® Anti-LAIR1 antibody [NKTA255]

Flow Cytometry - PerCP/Cy5.5® Anti-LAIR1 antibody [NKTA255] (AB234263)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB267788

Anti-Poliovirus Receptor/PVR antibody [EPR22672-151]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (AB267788)

  • 0
  • 0
Primary Antibodies

AB64693

Anti-Mannose Receptor antibody

BOND RX™ Validated

Western blot - Anti-Mannose Receptor antibody (AB64693)

  • 5
  • 42

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com