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AB323436

Alexa Fluor® 647 Anti-SLC16A3/MCT 4 antibody [EPR28177-30]

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Rabbit Recombinant Monoclonal SLC16A3/MCT 4 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

MCT3, MCT4, SLC16A3, Monocarboxylate transporter 4, MCT 4, Solute carrier family 16 member 3

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB323436)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB323436)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling SLC16A3/MCT 4 with ab323436 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing membranous staining in PC-3 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : K-562 (PMID : 25957999)
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-SLC16A3/MCT 4 antibody [EPR28177-30]

  • Unconjugated

    Anti-SLC16A3/MCT 4 antibody [EPR28177-30]

  • Carrier free

    Anti-SLC16A3/MCT 4 antibody [EPR28177-30] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28177-30

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC16A3 also known as MCT4 or MCT-4 is a member of the monocarboxylate transporter family. It is responsible for the transport of lactate and other monocarboxylates across cell membranes. Typically the molecular weight of MCT4 is approximately 45-50 kDa. This transporter is most abundant in tissues with high glycolytic activity including muscle and certain types of tumors where it facilitates the export of lactate produced during anaerobic metabolism to maintain intracellular pH balance and energy production efficiency.
Biological function summary

The transport function of SLC16A3/MCT4 is important for maintaining the energy equilibrium in cells that rely heavily on glycolysis. MCT4 does not function independently; it associates with ancillary proteins like CD147/Basigin which is essential for its correct localization to the plasma membrane. This association enhances the transport efficiency of lactate making MCT4 an important player in the lactate shuttle system a mechanism that allows for effective energy utilization in muscle fibers and tumor cells and significantly contributes to the regulation of the tissue microenvironment.

Pathways

SLC16A3/MCT4 plays a significant role in metabolic pathways particularly the glycolytic pathway by facilitating lactate efflux. The transporter is also implicated in the Cori cycle where lactate is shuttled from muscles to the liver for gluconeogenesis. Its functions overlap with those of other monocarboxylate transporters such as MCT1 which generally handles the import of lactate in oxidative tissues. Together these transporters maintain homeostasis of lactate levels in various tissues preventing lactate accumulation.

Alterations in SLC16A3/MCT4 expression or function are associated with cancer and anaerobic exercise intolerance. In cancer overexpression of MCT4 correlates with aggressive tumor behavior due to its role in lactate export aiding in the acidic tumor microenvironment development which favors cancer cell survival and invasion. Additionally interactions with proteins like CD147 are thought to modulate tumor progression. In exercise intolerance impaired lactate transport leads to muscle fatigue and reduced exercise capacity highlighting the critical role MCT4 plays in normal metabolic responses during intense physical activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proton-dependent transporter of monocarboxylates such as L-lactate and pyruvate (PubMed : 11101640, PubMed : 23935841, PubMed : 31719150). Plays a predominant role in L-lactate efflux from highly glycolytic cells (By similarity).
See full target information SLC16A3
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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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