Alexa Fluor® 647 Anti-SLC34A2 - N-terminal antibody [SP322]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-SLC34A2 - N-terminal antibody [SP322] (AB315179)

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling SLC34A2 - N-terminal with ab315179 at 1/100 (5.0 ug/ml).

Membranous staining on human endometrium. The section was incubated with ab315179 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-SLC34A2 - N-terminal antibody [SP322] (AB315179)

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling SLC34A2 - N-terminal with ab315179 at 1/100 (5.0 ug/ml).

Membranous staining on human lung cancer. The section was incubated with ab315179 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SLC34A2 - N-terminal antibody [SP322] (AB315179)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH : OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labelling SLC34A2 - N-terminal with ab315179 at 1/50 (10.0 ug/ml) dilution. Confocal image showing cytoplasmic and membranous staining in NIH : OVCAR-3 cells. Negative control : A549 (PMID 25017204). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Green). The Nuclear counterstain was DAPI (Blue).