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AB198367

Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

5

(3 Reviews)

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(8 Publications)

Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker - Alexa Fluor® 647 conjugated (ab198367) is a rabbit recombinant monoclonal antibody detecting Sodium Potassium ATPase in ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB198367)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB198367)

ab198367 staining Sodium Potassium ATPase in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198367 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • Biotin

    Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • HRP

    HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control

  • 578 PE

    PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • 660 APC

    APC Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • Unconjugated

    Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control

  • Carrier free

    Anti-Sodium Potassium ATPase antibody [EP1845Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1845Y

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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AB58475

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Tilapia": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (ab198367) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EP1845Y is also available pre-conjugated to a variety of labels for your convenience – Anti-Sodium Potassium ATPase Alexa Fluor® 647 [EP1845Y] - Plasma Membrane Marker (ab198367).

Other related products
We have a range of other formats of antibody clone [EP1845Y] also available for your convenience: ab76020, Carrier free - ab167390, HRP - ab185065, Alexa Fluor® 488 - ab197713, Biotin - ab198366, Alexa Fluor® 647 - ab198367, PE - ab209299, Alexa Fluor® 405 - ab210143, Alexa Fluor® 555 - ab274883, ab283318, Carrier free - ab283340, Carrier free - ab283341, ab283345, APC - ab314288

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium Potassium ATPase also known as Na/K-ATPase or NaK Pump is an essential enzyme that performs active transport of sodium (Na+) and potassium (K+) ions across the plasma membrane. This transmembrane protein has a molecular weight typically ranging from 100 to 120 kDa. It is expressed in most cell types particularly abundant in nerve and muscle cells. The ATPase hydrolyzes ATP to drive the exchange of three intracellular sodium ions for two extracellular potassium ions maintaining vital gradients across the cell membrane.
Biological function summary

The Na/K ATPase plays an important role in maintaining cellular homeostasis and membrane potential. It is an integral component of the cell membrane often operating as part of larger multiprotein complexes. This pump is responsible for providing the driving force behind secondary transport systems by maintaining ionic gradients. The presence of Na/K ATPase is also important for the functioning of the nervous system as it helps propagate action potentials in neurons.

Pathways

The Na/K ATPase is integral to the regulation of cardiac contraction and nerve signal transmission. It participates in pathways like the cardiac glycoside pharmacodynamics and the sodium ion transport pathway. It interacts with proteins such as ankyrin which link the ATPase to the cellular cytoskeleton facilitating its distribution and stability within the cell membrane. These interactions are significant for ensuring precise ion homeostasis and efficient cellular operation.

Na/K ATPase is linked to conditions such as hypertension and congestive heart failure. These diseases are associated with improper functioning or regulation of the ATPase which affects sodium and potassium balance and blood pressure regulation. The target also interacts with the protein PLM (phospholemman) which modulates its activity under different physiological and pathological conditions. Malfunction or mutations in the genes encoding Na/K ATPase subunits can contribute to neurological and cardiovascular disorders making it a therapeutic target in associated treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed : 29499166, PubMed : 30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).
See full target information ATP1A1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:1062 PubMed39215205

2024

UNSEG: unsupervised segmentation of cells and their nuclei in complex tissue samples.

Applications

Unspecified application

Species

Unspecified reactive species

Bogdan Kochetov,Phoenix D Bell,Paulo S Garcia,Akram S Shalaby,Rebecca Raphael,Benjamin Raymond,Brian J Leibowitz,Karen Schoedel,Rhonda M Brand,Randall E Brand,Jian Yu,Lin Zhang,Brenda Diergaarde,Robert E Schoen,Aatur Singhi,Shikhar Uttam

Cell 186:5620-5637.e16 PubMed38065082

2023

Molecular cartography uncovers evolutionary and microenvironmental dynamics in sporadic colorectal tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Cody N Heiser,Alan J Simmons,Frank Revetta,Eliot T McKinley,Marisol A Ramirez-Solano,Jiawei Wang,Harsimran Kaur,Justin Shao,Gregory D Ayers,Yu Wang,Sarah E Glass,Naila Tasneem,Zhengyi Chen,Yan Qin,William Kim,Andrea Rolong,Bob Chen,Paige N Vega,Julia L Drewes,Nicholas O Markham,Nabil Saleh,Fotis Nikolos,Simon Vandekar,Angela L Jones,M Kay Washington,Joseph T Roland,Keith S Chan,Thomas Schürpf,Cynthia L Sears,Qi Liu,Martha J Shrubsole,Robert J Coffey,Ken S Lau

Cellular and molecular life sciences : CMLS 79:592 PubMed36378343

2022

CFTR modulates aquaporin-mediated glycerol permeability in mouse Sertoli cells.

Applications

Unspecified application

Species

Unspecified reactive species

João C Ribeiro,Raquel L Bernardino,David F Carrageta,Graça Soveral,Giuseppe Calamita,Marco G Alves,Pedro F Oliveira

Cancers 14: PubMed35406554

2022

Targeting GLI1 Transcription Factor for Restoring Iodine Avidity with Redifferentiation in Radioactive-Iodine Refractory Thyroid Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Min Oh,Ramya Lakshmi Rajendran,Prakash Gangadaran,Chae Moon Hong,Ju Hye Jeong,Jaetae Lee,Byeong-Cheol Ahn

Cell 184:6262-6280.e26 PubMed34910928

2021

Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Bob Chen,Cherie' R Scurrah,Eliot T McKinley,Alan J Simmons,Marisol A Ramirez-Solano,Xiangzhu Zhu,Nicholas O Markham,Cody N Heiser,Paige N Vega,Andrea Rolong,Hyeyon Kim,Quanhu Sheng,Julia L Drewes,Yuan Zhou,Austin N Southard-Smith,Yanwen Xu,James Ro,Angela L Jones,Frank Revetta,Lynne D Berry,Hiroaki Niitsu,Mirazul Islam,Karin Pelka,Matan Hofree,Jonathan H Chen,Siranush Sarkizova,Kimmie Ng,Marios Giannakis,Genevieve M Boland,Andrew J Aguirre,Ana C Anderson,Orit Rozenblatt-Rosen,Aviv Regev,Nir Hacohen,Kenta Kawasaki,Toshiro Sato,Jeremy A Goettel,William M Grady,Wei Zheng,M Kay Washington,Qiuyin Cai,Cynthia L Sears,James R Goldenring,Jeffrey L Franklin,Timothy Su,Won Jae Huh,Simon Vandekar,Joseph T Roland,Qi Liu,Robert J Coffey,Martha J Shrubsole,Ken S Lau

Nature cell biology 23:1240-1254 PubMed34887515

2021

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Zhang,Dennis K Jeppesen,James N Higginbotham,Ramona Graves-Deal,Vincent Q Trinh,Marisol A Ramirez,Yoojin Sohn,Abigail C Neininger,Nilay Taneja,Eliot T McKinley,Hiroaki Niitsu,Zheng Cao,Rachel Evans,Sarah E Glass,Kevin C Ray,William H Fissell,Salisha Hill,Kristie Lindsey Rose,Won Jae Huh,Mary Kay Washington,Gregory Daniel Ayers,Dylan T Burnette,Shivani Sharma,Leonard H Rome,Jeffrey L Franklin,Youngmin A Lee,Qi Liu,Robert J Coffey

Aging 12: PubMed33281118

2020

Asiatic acid protects oocytes against aging-induced deterioration and improves subsequent embryonic development in pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Yi Hu,Xiao Xia Li,Yun Fei Diao,Jia-Jia Qi,Da-Li Wang,Jia-Bao Zhang,Bo-Xing Sun,Shuang Liang

PloS one 13:e0189464 PubMed29642240

2018

Sodium bicarbonate cotransporter NBCe2 gene variants increase sodium and bicarbonate transport in human renal proximal tubule cells.

Applications

WB

Species

Unspecified reactive species

John J Gildea,Peng Xu,Brandon A Kemp,Julia M Carlson,Hanh T Tran,Dora Bigler Wang,Christophe J Langouët-Astrié,Helen E McGrath,Robert M Carey,Pedro A Jose,Robin A Felder
View all publications
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