AB207677

Alexa Fluor® 647 Anti-SOX9 antibody [EPR14335-78]

Name: Alexa Fluor® 647 conjugated Anti-SOX9 antibody [EPR14335-78] (ab207677) | Abcam
Brand: Abcam Limited
SKU: AB207677
Price: 620 USD
Availability: InStock
Rating: 5 (8 reviews)

RabMAb
Recombinant
What is this?

(8 Reviews)

(3 Publications)

Anti-SOX9 antibody [EPR14335-78] - Alexa Fluor® 647 conjugated (ab207677) is a rabbit recombinant monoclonal antibody detecting SOX9 in ICC/IF. Suitable for Mouse.

- Clone EPR14335-78 is the most cited clone to SOX9
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Transcription factor SOX-9, SOX9

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SOX9 antibody [EPR14335-78] (AB207677)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SOX9 antibody [EPR14335-78] (AB207677)

ab207677 staining SOX9 in Neuro-2a cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207677 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Carrier free

Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free
Unconjugated

Anti-SOX9 antibody [EPR14335-78]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-SOX9 antibody [EPR14335-78]
578 PE

PE Anti-SOX9 antibody [EPR14335-78]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14335-78

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Mouse, Human, Mouse, Rat

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "This product gave a positive signal in PC-3 cells fixed with 4% formaldehyde (10 min)", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-SOX9 antibody [EPR14335-78] (ab207677) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

Reviewed by scientists
Anti-SOX9 [EPR14335-78] Alexa Fluor® 647 (ab207677) has over 5 independent reviews from customers.

Related products
Antibody clone EPR14335-78 is also available pre-conjugated to a variety of labels for your convenience – Anti-SOX9 Alexa Fluor® 647 [EPR14335-78] (ab207677).

Other related products
We have a range of other formats of antibody clone [EPR14335-78] also available for your convenience: ab185966, Alexa Fluor® 647 - ab207677, Alexa Fluor® 488 - ab208427, PE - ab224019, Carrier free - ab225541, Alexa Fluor® 790 - ab309324

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that plays a key role in chondrocytes differentiation and skeletal development (PubMed : 24038782). Specifically binds the 5'-ACAAAG-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including cartilage matrix protein-coding genes COL2A1, COL4A2, COL9A1, COL11A2 and ACAN, SOX5 and SOX6 (PubMed : 8640233). Also binds to some promoter regions (By similarity). Plays a central role in successive steps of chondrocyte differentiation (By similarity). Absolutely required for precartilaginous condensation, the first step in chondrogenesis during which skeletal progenitors differentiate into prechondrocytes (By similarity). Together with SOX5 and SOX6, required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes, the second step in chondrogenesis (By similarity). Later, required to direct hypertrophic maturation and block osteoblast differentiation of growth plate chondrocytes : maintains chondrocyte columnar proliferation, delays prehypertrophy and then prevents osteoblastic differentiation of chondrocytes by lowering beta-catenin (CTNNB1) signaling and RUNX2 expression (By similarity). Also required for chondrocyte hypertrophy, both indirectly, by keeping the lineage fate of chondrocytes, and directly, by remaining present in upper hypertrophic cells and transactivating COL10A1 along with MEF2C (By similarity). Low lipid levels are the main nutritional determinant for chondrogenic commitment of skeletal progenitor cells : when lipids levels are low, FOXO (FOXO1 and FOXO3) transcription factors promote expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Mechanistically, helps, but is not required, to remove epigenetic signatures of transcriptional repression and deposit active promoter and enhancer marks at chondrocyte-specific genes (By similarity). Acts in cooperation with the Hedgehog pathway-dependent GLI (GLI1 and GLI3) transcription factors (By similarity). In addition to cartilage development, also acts as a regulator of proliferation and differentiation in epithelial stem/progenitor cells : involved in the lung epithelium during branching morphogenesis, by balancing proliferation and differentiation and regulating the extracellular matrix (By similarity). Controls epithelial branching during kidney development (By similarity).

See full target information Transcription factor SOX-9

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Archives of toxicology 98:2695-2709 PubMed38769170

2024

Mechanistic screening of reproductive toxicity in a novel 3D testicular co-culture model shows significant impairments following exposure to low-dibutyl phthalate concentrations.

Applications

Unspecified application

Species

Unspecified reactive species

Radwa Almamoun,Paula Pierozan,Oskar Karlsson

Cell 186:5910-5924.e17 PubMed38070509

2023

A tridimensional atlas of the developing human head.

Applications

IHC - Wmt

Species

Human

Raphael Blain,Gérard Couly,Eimad Shotar,Joséphine Blévinal,Maryne Toupin,Anais Favre,Ali Abjaghou,Megumi Inoue,Edwin Hernández-Garzón,Frédéric Clarençon,Frédéric Chalmel,Séverine Mazaud-Guittot,Paolo Giacobini,Yorick Gitton,Alain Chédotal

Biomolecules 12: PubMed36358914

2022

Semicarbazide-Sensitive Amine Oxidase (SSAO) and Lysyl Oxidase (LOX) Association in Rat Aortic Vascular Smooth Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vesna Manasieva,Shori Thakur,Lisa A Lione,Jessal Patel,Anwar Baydoun,John Skamarauskas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-SOX9 antibody [EPR14335-78]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SOX9 antibody [EPR14335-78] (AB207677)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

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Aliquoting information

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Product protocols

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Publications (3)

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