AB194721

Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

RabMAb
Recombinant
KO Validated
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(6 Publications)

Rabbit Recombinant Monoclonal SQSTM1 / p62 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

ORCA, OSIL, SQSTM1, Sequestosome-1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBIAP, p60, p62

4 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB194721)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB194721)

ab194721 staining SQSTM1 in wild-type HAP1 cells (top panel) and SQSTM1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab194721 at 1/500 (shown in red) and ab195887 at 1/250 dilution (shown in green). Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB194721)

ab194721 staining SQSTM1/p62 in HeLa cells +/- Chloroquine (50μM, 24 hours). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab194721 at 1/500 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB194721)

ab194721 staining SQSTM1/p62 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab194721 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 488, shown in green) at 1/167 dilution, overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB194721)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB194721)

Overlay histogram showing MCF7 cells stained with ab194721 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab194721, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.

This antibody gave a positive signal in MCF7 fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Carrier free

Anti-SQSTM1 / p62 antibody [EPR4844] - BSA and Azide free
Unconjugated

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker
660 APC

APC Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker
HRP

HRP Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker
578 PE

PE Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4844

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/alexa-fluor-647-rabbit-igg-monoclonal-epr25a-isotype-control-ab199093'>ab199093</a> - Rabbit monoclonal IgG (Alexa Fluor®; 647), is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes (PubMed : 15340068, PubMed : 15953362, PubMed : 16286508, PubMed : 17580304, PubMed : 20168092, PubMed : 22017874, PubMed : 22622177, PubMed : 24128730, PubMed : 28404643, PubMed : 29343546, PubMed : 29507397, PubMed : 31857589, PubMed : 33509017, PubMed : 34471133, PubMed : 34893540, PubMed : 35831301, PubMed : 37306101, PubMed : 37802024). Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps (PubMed : 16286508, PubMed : 20168092, PubMed : 22622177, PubMed : 24128730, PubMed : 28404643, PubMed : 29343546, PubMed : 29507397, PubMed : 34893540, PubMed : 37802024). SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies (PubMed : 15911346, PubMed : 20168092, PubMed : 22017874, PubMed : 24128730, PubMed : 29343546, PubMed : 29507397, PubMed : 31857589, PubMed : 37802024). SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins (PubMed : 16286508, PubMed : 17580304, PubMed : 20168092, PubMed : 22622177, PubMed : 24128730, PubMed : 28404643, PubMed : 37802024). SQSTM1 is itself degraded along with its ubiquitinated cargos (PubMed : 16286508, PubMed : 17580304, PubMed : 37802024). Also required to recruit ubiquitinated proteins to PML bodies in the nucleus (PubMed : 20168092). Also involved in autophagy of peroxisomes (pexophagy) in response to reactive oxygen species (ROS) by acting as a bridge between ubiquitinated PEX5 receptor and autophagosomes (PubMed : 26344566). Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1 : interaction inactivates the BCR(KEAP1) complex by sequestering the complex in inclusion bodies, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes (PubMed : 20452972, PubMed : 28380357, PubMed : 33393215, PubMed : 37306101). Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes (PubMed : 29496741). Involved in endosome organization by retaining vesicles in the perinuclear cloud : following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed : 27368102, PubMed : 33472082). Sequesters tensin TNS2 into cytoplasmic puncta, promoting TNS2 ubiquitination and proteasomal degradation (PubMed : 25101860). May regulate the activation of NFKB1 by TNF, nerve growth factor (NGF) and interleukin-1 (PubMed : 10356400, PubMed : 10747026, PubMed : 11244088, PubMed : 12471037, PubMed : 16079148, PubMed : 19931284). May play a role in titin/TTN downstream signaling in muscle cells (PubMed : 15802564). Adapter that mediates the interaction between TRAF6 and CYLD (By similarity).

See full target information SQSTM1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 26:4040-4071 PubMed40550954

2025

LRBA deficiency impairs autophagy and contributes to enhanced antigen presentation and T-cell dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Sindram,Marie-Celine Deau,Laure-Anne Ligeon,Pablo Sanchez-Martin,Sigrun Nestel,Sophie Jung,Stefanie Ruf,Pankaj Mishra,Michele Proietti,Stefan Günther,Kathrin Thedieck,Eleni Roussa,Angelika Rambold,Christian Münz,Claudine Kraft,Bodo Grimbacher,Laura Gámez-Díaz

Scientific reports 13:6393 PubMed37076504

2023

Dentin defects caused by a Dspp frameshift mutation are associated with the activation of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Liang,Charles E Smith,Yuanyuan Hu,Hong Zhang,Chuhua Zhang,Qian Xu,Yongbo Lu,Ling Qi,Jan C-C Hu,James P Simmer

Cell stem cell 30:460-472.e6 PubMed36948186

2023

Hematopoietic stem cells preferentially traffic misfolded proteins to aggresomes and depend on aggrephagy to maintain protein homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Bernadette A Chua,Connor J Lennan,Mary Jean Sunshine,Daniela Dreifke,Ashu Chawla,Eric J Bennett,Robert A J Signer

Cells 10: PubMed34572117

2021

An Autophagy Modulator Peptide Prevents Lung Function Decrease and Corrects Established Inflammation in Murine Models of Airway Allergy.

Applications

Unspecified application

Species

Unspecified reactive species

François Daubeuf,Nicolas Schall,Nathalie Petit-Demoulière,Nelly Frossard,Sylviane Muller

eLife 10: PubMed34396954

2021

HIF1α is required for NK cell metabolic adaptation during virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco Victorino,Tarin M Bigley,Eugene Park,Cong-Hui Yao,Jeanne Benoit,Li-Ping Yang,Sytse J Piersma,Elvin J Lauron,Rebecca M Davidson,Gary J Patti,Wayne M Yokoyama

Cell reports 30:687-698.e6 PubMed31968246

2020

Human Liver Memory CD8 T Cells Use Autophagy for Tissue Residence.

Applications

Unspecified application

Species

Unspecified reactive species

Leo Swadling,Laura J Pallett,Mariana O Diniz,Josephine M Baker,Oliver E Amin,Kerstin A Stegmann,Alice R Burton,Nathalie M Schmidt,Anna Jeffery-Smith,Nekisa Zakeri,Kornelija Suveizdyte,Farid Froghi,Giuseppe Fusai,William M Rosenberg,Brian R Davidson,Anna Schurich,A Katharina Simon,Mala K Maini

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB194721)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB194721)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB194721)

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB194721)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

Purification technique

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Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise