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AB319636

Alexa Fluor® 647 Anti-Stromal interaction molecule 1 antibody [EPR3414]

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Rabbit Recombinant Monoclonal Stromal interaction molecule 1 antibody - conjugated to Alexa Fluor® 647.

View Alternative Names

GOK, STIM1, Stromal interaction molecule 1

  • 660 APC

    APC Anti-Stromal interaction molecule 1 antibody [EPR3414]

  • 578 PE

    PE Anti-Stromal interaction molecule 1 antibody [EPR3414]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Stromal interaction molecule 1 antibody [EPR3414]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Stromal interaction molecule 1 antibody [EPR3414]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Stromal interaction molecule 1 antibody [EPR3414]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Stromal interaction molecule 1 antibody [EPR3414]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3414

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Stromal interaction molecule 1 also known as STIM1 is a single-pass transmembrane protein primarily involved in the regulation of calcium ion entry into cells. It acts as a sensor of calcium levels inside the endoplasmic reticulum. STIM1 is about 90 kDa in mass and expresses in various tissues including the immune system skeletal muscle and vascular smooth muscle. The protein anchors in the membrane of the endoplasmic reticulum where it responds to the depletion of calcium by communicating with plasma membrane channels to activate calcium influx.
Biological function summary

STIM1 plays a critical role in the store-operated calcium entry (SOCE) mechanism a significant process in cellular calcium signaling. STIM1 senses calcium concentration within the endoplasmic reticulum and upon depletion aggregates and migrates to the plasma membrane to interact with Orai1 part of the calcium release-activated calcium (CRAC) channel complex. This interaction opens the channel allowing calcium ions to flow into the cytosol which is necessary for various cellular functions such as gene expression and cell proliferation.

Pathways

STIM1 functions integrally in the calcium signaling pathway a system central to numerous physiological processes. Here STIM1 ensures appropriate calcium ion concentrations facilitate activation of various transcription factors and enzymes. It closely associates with Orai1 triggering the CRAC channel activation in response to the endoplasmic reticulum calcium levels. Additionally STIM1 interacts with other signaling molecules like protein kinase C coordinating between different pathways to maintain cellular homeostasis.

STIM1 dysfunction links to conditions such as immunodeficiency and muscle disorders. Mutations in STIM1 can lead to combined immunodeficiency characterized by impaired immune responses due to faulty calcium influx in lymphocytes. Additionally aberrations in STIM1 activity associate with tubular aggregate myopathy a muscle disorder where abnormal calcium handling in muscle cells leads to muscle weakness and pain. In these diseases the interaction between STIM1 and Orai1 is often disrupted highlighting their roles in maintaining physiological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a Ca(2+) sensor that gates two major inward rectifying Ca(2+) channels at the plasma membrane : Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels (PubMed : 15866891, PubMed : 16005298, PubMed : 16208375, PubMed : 16537481, PubMed : 16733527, PubMed : 16766533, PubMed : 16807233, PubMed : 18854159, PubMed : 19182790, PubMed : 19249086, PubMed : 19622606, PubMed : 19706554, PubMed : 22464749, PubMed : 24069340, PubMed : 24351972, PubMed : 24591628, PubMed : 25326555, PubMed : 26322679, PubMed : 28219928, PubMed : 32415068). Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates CRAC channel pore-forming subunits ORA1, ORA2 and ORAI3 to generate sustained and oscillatory Ca(2+) entry (PubMed : 16208375, PubMed : 16537481, PubMed : 32415068). Involved in enamel formation (PubMed : 24621671).
See full target information STIM1
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Complementary Products

Proteins & Peptides

AB78681

Recombinant Human Stromal interaction molecule 1 protein

SDS-PAGE - Recombinant Human Stromal interaction molecule 1 protein (AB78681)

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