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AB305212

Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker

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Rabbit Recombinant Monoclonal Synapsin I antibody - conjugated to Alexa Fluor® 647. Synapse marker. Suitable for IHC-Fr, ICC/IF and reacts with Mouse, Rat samples.

View Alternative Names

Syn-1, Syn1, Synapsin-1, Synapsin I

6 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB305212)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB305212)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung (fresh) tissue labeling Synapsin I with ab305212 at 1/500 (1.0 ug/ml) dilution. Negative control : confocal image showing no staining on mouse lung tissue (PMID : 9539796). The nuclear counterstain was DAPI (Blue). The section was incubated with ab305212 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on aLeica Biosystems BOND® RX instrument. Image was taken with a confocalmicroscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB305212)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB305212)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung (fresh) tissue labeling Synapsin I with ab305212 at 1/500 (1.0 ug/ml) dilution. Negative control : confocal image showing no staining on rat lung tissue (PMID : 9539796). The nuclear counterstain was DAPI (Blue). The section was incubated with ab305212 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on aLeica Biosystems BOND® RX instrument. Image was taken with a confocalmicroscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB305212)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB305212)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh) tissue labeling Synapsin I with ab305212 at 1/500 (1.0 ug/ml) dilution. Confocal image showing positive staining on mouse hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab305212 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Negative control : PBS instead of the primary antibody.

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB305212)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB305212)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh) tissue labeling Synapsin I with ab305212 at 1/500 (1.0 ug/ml) dilution. Confocal image showing positive staining on rat hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab305212 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Negative control : PBS instead of the primary antibody.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB305212)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB305212)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Synapsin I with ab305212 at 1/100 (5.0 ug/ml) dilution. Confocal image showing positive staining in rat primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : PBS instead of the primary antibody.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB305212)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB305212)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Synapsin I with ab305212 at 1/100 (5.0 ug/ml) dilution. Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : PBS instead of the primary antibody.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Synapsin I - Synaptic Marker antibody [EPR23531-50]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Synapsin I - Synaptic Marker antibody [EPR23531-50]

  • 578 PE

    PE Anti-Synapsin I antibody [EPR23531-50] – Synaptic Marker

  • 660 APC

    APC Anti-Synapsin I antibody [EPR23531-50] – Synaptic Marker

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker

  • Unconjugated

    Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker

  • Carrier free

    Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23531-50

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-Fr, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synapsin I also known as SYN1 plays an important role in synaptic function. It is a phosphoprotein with a molecular mass of approximately 78 kDa. Synapsin I is expressed mainly in the neurons of the central nervous system (CNS). It binds to synaptic vesicles and actin cytoskeleton which suggests that it functions in modulating neurotransmitter release at the presynaptic terminals. This modulation occurs as synapsin I undergoes phosphorylation which is critical for its activity.
Biological function summary

Synapsin I influences synaptic plasticity and is part of the synaptic vesicle trafficking complex. In its dephosphorylated state Synapsin I associates with synaptic vesicles anchoring them to the actin cytoskeleton. Upon phosphorylation Synapsin I changes conformation causing vesicles to mobilize. This activity supports the modulation of neurotransmitter release impacting learning and memory functions.

Pathways

Synapsin I participates significantly in the neurotransmitter release cycle and synaptic vesicle trafficking pathway. Protein kinase A (PKA) and Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulate its phosphorylation affecting how Synapsin I contributes to vesicle release. The phosphorylation of Synapsin I at sites such as serine 9 enables its interaction with other proteins like actin and spectrin facilitating vesicle movement.

Altered Synapsin I expression associates with neurological conditions like epilepsy and schizophrenia. In epilepsy dysregulation of Synapsin I phosphorylation processes can result in imbalanced neurotransmitter release potentially leading to seizures. Its connection to schizophrenia involves changes in synaptic plasticity which neurotransmitter systems such as dopamine and related proteins like alpha-synuclein also influence. Understanding these interactions can aid in developing therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (By similarity). Also involved in the regulation of axon outgrowth and synaptogenesis (PubMed : 7568107). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level (By similarity).
See full target information Syn1
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Product promise

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For full details, please see our Terms & Conditions

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