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Rabbit Recombinant Monoclonal Syntaxin 3 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Syntaxin 3 antibody [EPR8543] (AB314933), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Syntaxin 3 antibody [EPR8543] (AB314933), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Syntaxin 3 antibody [EPR8543] (AB314933), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Syntaxin 3 antibody [EPR8543] (AB314933), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Human

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

4 products for Alternative Version

    Target data

    Function

    Potentially involved in docking of synaptic vesicles at presynaptic active zones.

    Alternative names

    Syntaxin-3, STX3A, STX3

    Recommended products

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    Rabbit Recombinant Monoclonal Syntaxin 3 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

    Alternative names

    Syntaxin-3, STX3A, STX3

    Key facts

    Isotype

    IgG

    Conjugation

    Alexa Fluor® 647

    Excitation/Emission

    Ex: 650nm, Em: 665nm

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Clone number

    EPR8543

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle, Store in the dark

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Supplementary info

    Activity summary

    Syntaxin 3 sometimes known as Stx3 is a protein involved in vesicle trafficking and membrane fusion. It plays a mechanical role by facilitating the fusion of transport vesicles with target membranes an essential step in intracellular transport mechanisms. Syntaxin 3 is a member of the t-SNARE family and has a molecular mass of approximately 33 kDa. This protein expresses mainly in epithelial cells including those in the kidney and lungs as well as certain neuronal tissues indicating its involvement in critical cellular processes in these areas.

    Biological function summary

    Syntaxin 3 acts in cellular transport by participating in the trans-Golgi network-to-plasma membrane pathway. This protein is part of the SNARE complex a group of proteins that mediates vesicle fusion. By engaging with other SNARE proteins like SNAP-25 and VAMP Syntaxin 3 helps ensure the precise delivery of vesicle contents to the plasma membrane. Its role is important for maintaining cellular homeostasis by regulating how cells communicate and exchange materials with their environment.

    Pathways

    Syntaxin 3 is an integral component of the exocytosis pathway and is involved in the secretory pathway. In exocytosis Syntaxin 3 associates with proteins such as Munc18 and synaptotagmin which help in vesicle docking and fusion. In the secretory pathway Syntaxin 3 is responsible for directing proteins and lipids to their intended membrane sites thereby influencing cellular communication. These interactions guide the flow of proteins within vesicle transport networks and coordinate various signaling events within the cell.

    Associated diseases and disorders

    Syntaxin 3 has connections to conditions such as cystic fibrosis and certain types of diabetes. In cystic fibrosis abnormal function of Syntaxin 3 may disrupt normal secretory processes in epithelial cells contributing to enhanced pathology. The protein is associated with the cystic fibrosis transmembrane conductance regulator (CFTR) protein which fails to localize correctly in this disease. In diabetes Syntaxin 3 impacts insulin secretion due to its role in pancreatic beta cells tying it to the disorder through insulin-regulating mechanisms with proteins such as GLUT4.

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    4 product images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Syntaxin 3 antibody [EPR8543] (ab314933), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Syntaxin 3 antibody [EPR8543] (ab314933)

      Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling Syntaxin 3 with ab314933 at 1/50 (10.0 ug/ml).
      Negative control: no staining on human skeletal muscle.
      The section was incubated with ab314933 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Syntaxin 3 antibody [EPR8543] (ab314933), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Syntaxin 3 antibody [EPR8543] (ab314933)

      Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Syntaxin 3 with ab314933 at 1/50 (10.0 ug/ml).
      Apical cell membranous staining on rat kidney.
      The section was incubated with ab314933 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Syntaxin 3 antibody [EPR8543] (ab314933), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Syntaxin 3 antibody [EPR8543] (ab314933)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Syntaxin 3 with ab314933 at 1/50 (10.0 ug/ml).
      Apical cell membranous staining on mouse kidney.
      The section was incubated with ab314933 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Syntaxin 3 antibody [EPR8543] (ab314933), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Syntaxin 3 antibody [EPR8543] (ab314933)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Syntaxin 3 with ab314933 at 1/50 (10.0 ug/ml).
      Apical cell membranous staining on human kidney.
      The section was incubated with ab314933 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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