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Mouse Monoclonal TAU antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr and reacts with Human samples.

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Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (AB317764), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (AB317764), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-Fr
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Do not perform antigen retrieval.

Target data

Function

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Recommended products

Mouse Monoclonal TAU antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr and reacts with Human samples.

Key facts

Isotype
IgG1
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
GT-38
Purification technique
Affinity purification Protein G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tau protein also known as MAPT (Microtubule-Associated Protein Tau) plays a mechanical role in stabilizing microtubules in neuronal axons. The human tau protein has several isoforms generally weighing between 45 to 65 kDa. Tau is predominantly expressed in neurons of the central nervous system but can also be found in some non-neuronal cells. In neurons tau binds to tubulin and facilitates the polymerization and stability of microtubules which are essential for maintaining cellular structure and transport functions.

Biological function summary

Tau protein impacts the dynamics of the neuronal cytoskeleton. Tau is part of the intracellular transport system where it facilitates the movement of organelles and proteins along axons. This function is essential for neuron communication and resilience. Tau interacts with and regulates microtubule-associated complexes ensuring efficient intracellular transport. Its role in cytoskeletal integrity strongly influences cell signaling pathways critical for synaptic plasticity and neuronal survival.

Pathways

Tau protein plays a significant part in cellular transport and signal transduction pathways. One key pathway is the MAPK signaling pathway where tau's phosphorylation state influences neuronal response to external stimuli. Additionally tau is involved in the PI3K/AKT pathway affecting cell growth and survival. Within these pathways tau's phosphorylation is regulated by kinases including GSK-3β and CDK5 while phosphatases like PP2A dephosphorylate tau to maintain its functional state.

Associated diseases and disorders

Dysfunctional tau is strongly linked to neurodegenerative diseases particularly Alzheimer's disease (AD) and frontotemporal dementia (FTD). In AD hyperphosphorylated tau forms neurofibrillary tangles which disrupt neuronal function and promote cell death. This process often involves other proteins such as beta-amyloid which exacerbate tau's pathological aggregation. Understanding tauopathy mechanisms has guided the research and development of anti-tau antibodies like anti-GT3 and anti-conformation antibodies as therapeutic strategies against tau-related pathologies in neurodegenerative disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab317764), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab317764)

    Immunofluorescence staining of Tau Alzheimers Disease in a section of frozen human Alzheimer brain*.

    The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BONDTM. The section was incubated at room temperature for 1 hour with ab317764 at 1/50 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab317764), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab317764)

    Negative immunofluorescence staining of Tau Alzheimers Disease in a section of frozen human normal brain*.

    The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BONDTM. The section was incubated at room temperature for 1 hour with ab317764 at 1/50 dilution. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com