Rabbit Recombinant Monoclonal TCF-4 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
Alexa Fluor® 647
Ex: 650nm, Em: 665nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
Flow Cyt (Intra) | |
---|---|
Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
Select an associated product type
Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
BHLHB19, ITF2, SEF2, ITF2, BHLHB19, TCF4, SEF2, Transcription factor 4, TCF-4, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2, bHLHb19, ITF-2, SEF-2
Rabbit Recombinant Monoclonal TCF-4 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
Alexa Fluor® 647
Ex: 650nm, Em: 665nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
NCI-R159-6
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
This supplementary information is collated from multiple sources and compiled automatically.
The transcription factor 4 (TCF-4) also known as TCF7L2 is an important regulator of transcriptional activity. This protein weighs approximately 68 kDa and is widely expressed in multiple tissues including the pancreas intestine and brain. Through its function as a transcription factor TCF-4 binds to specific DNA sequences influencing the expression of target genes. The regulation it provides is essential for various biological processes especially during developmental stages.
TCF-4 plays an important role in Wnt signaling a pathway critical for cell growth and differentiation. It partners with β-catenin to form a functional complex enabling it to activate gene expression. This interaction influences cellular processes such as cell cycle regulation fate determination and maintenance of stem cell populations. As such TCF-4 serves an important role in regulating a balance between proliferation and differentiation in developing tissues.
TCF-4 is an important component within the canonical Wnt/β-catenin signaling pathway. This pathway is pivotal in embryogenesis and is also involved in the control of cell communication processes. TCF-4 interacts with other pathway members such as the APC protein which are essential for stabilizing β-catenin enabling successful transcriptional regulation. Its activity connects it chemically to the TCF/LEF family of proteins which all participate actively in gene transcription related to Wnt signaling.
TCF-4 has been implicated in type 2 diabetes and certain forms of cancer. Research shows that mutations in the TCF7L2 gene alter its function leading to increased susceptibility to type 2 diabetes emphasizing its role in glucose metabolism. Moreover dysregulation of the Wnt signaling pathway in which TCF-4 is an actor has been associated with cancer pathogenesis including colorectal cancer. TCF-4 connects closely with β-catenin and APC in these conditions highlighting its involvement in tumorigenic processes when its regulation fails.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Overlay histogram showing SH-SY5Y cells stained with ab246763 (red line). The cells were fixed with 80 % methanol (5 min) and then permeabilized with 0.1 % PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab246763) (1x 106 in 100μl at 0.1 μg/ml (1/5000)) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in SH-SY5Y cells fixed with 4 % formaldehyde (10 min) / permeabilized with 0.1 % PBS-Triton X-100 for 15 min used under the same conditions.
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