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Rabbit Recombinant Monoclonal TCF-4 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TCF-4 antibody [NCI-R159-6] (AB246763), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)
Human
Tested

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TCF-4 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

NCI-R159-6

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The transcription factor 4 (TCF-4) also known as TCF7L2 is an important regulator of transcriptional activity. This protein weighs approximately 68 kDa and is widely expressed in multiple tissues including the pancreas intestine and brain. Through its function as a transcription factor TCF-4 binds to specific DNA sequences influencing the expression of target genes. The regulation it provides is essential for various biological processes especially during developmental stages.

Biological function summary

TCF-4 plays an important role in Wnt signaling a pathway critical for cell growth and differentiation. It partners with β-catenin to form a functional complex enabling it to activate gene expression. This interaction influences cellular processes such as cell cycle regulation fate determination and maintenance of stem cell populations. As such TCF-4 serves an important role in regulating a balance between proliferation and differentiation in developing tissues.

Pathways

TCF-4 is an important component within the canonical Wnt/β-catenin signaling pathway. This pathway is pivotal in embryogenesis and is also involved in the control of cell communication processes. TCF-4 interacts with other pathway members such as the APC protein which are essential for stabilizing β-catenin enabling successful transcriptional regulation. Its activity connects it chemically to the TCF/LEF family of proteins which all participate actively in gene transcription related to Wnt signaling.

Associated diseases and disorders

TCF-4 has been implicated in type 2 diabetes and certain forms of cancer. Research shows that mutations in the TCF7L2 gene alter its function leading to increased susceptibility to type 2 diabetes emphasizing its role in glucose metabolism. Moreover dysregulation of the Wnt signaling pathway in which TCF-4 is an actor has been associated with cancer pathogenesis including colorectal cancer. TCF-4 connects closely with β-catenin and APC in these conditions highlighting its involvement in tumorigenic processes when its regulation fails.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TCF-4 antibody [NCI-R159-6] (ab246763), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TCF-4 antibody [NCI-R159-6] (ab246763)

    Overlay histogram showing SH-SY5Y cells stained with ab246763 (red line). The cells were fixed with 80 % methanol (5 min) and then permeabilized with 0.1 % PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab246763) (1x 106 in 100μl at 0.1 μg/ml (1/5000)) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in SH-SY5Y cells fixed with 4 % formaldehyde (10 min) / permeabilized with 0.1 % PBS-Triton X-100 for 15 min used under the same conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com