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AB317716

Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16]

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Rabbit Recombinant Monoclonal TRDC antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), Flow Cyt and reacts with Transfected cell line - Human, Human samples.

View Alternative Names

T cell receptor delta constant, TRDC

3 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (AB317716)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (AB317716)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype / 293T (human embryonic kidney epithelial cell) transfected with a TRDC (human TCR delta) protein expression vector containing a myc-His tag(Up Right) / 293T transfected with a TRGC1 protein (human TCR gamma) expression vector(Down Left) / 293T transfected with an empty vector containing a myc-His tag(Down Right) cells labelling TCR delta with ab317716 at 1/500 dilution (0.1ug)/ Up Right, Down Left and Down Right (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cytometry - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (AB317716)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (AB317716)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling TCR delta with ab317716 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells.Cells were co-stained with anti-CD3 conjugated to BV421.

Flow Cytometry - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (AB317716)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (AB317716)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling TCR delta with ab317716 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells.Cells were co-stained with anti-CD4 conjugated to BV421.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27043-16

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" }, "Transfected cell line - Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCR delta also known as T cell receptor delta chain is a component of the T-cell receptor complex found on gamma delta T cells. It has a molecular weight of approximately 60 kDa. TCR delta is expressed mainly in the thymus peripheral blood and epithelium. In the structure of TCR the delta chain pairs with the gamma chain to form a distinct type of TCR which differs from the more familiar alpha-beta TCR found on most T cells.
Biological function summary

TCR delta contributes to the immune response through recognition of antigens presented by non-classical MHC molecules such as those on stressed infected or transformed cells. It is an important player in the immune system with its expression often associated with mechanisms of immune surveillance and defense. TCR delta is part of a complex with CD3 proteins which are necessary for signal transduction leading to T cell activation. This chain together with the gamma chain enables recognition of a wide array of antigens contributing to immunity beyond typical peptide-dominated responses.

Pathways

Gamma delta T cells act in the immune response by recognizing stress-induced molecules and initiating protective reactions. TCR delta participates in signaling pathways that control T cell development and maturation including the MAPK and NF-κB pathways. These pathways involve interactions with proteins such as ERK JNK and IκB playing roles in the expansion and functional capabilities of T cells. The ability to recognize non-peptide antigens through TCR delta makes these pathways distinct in comparison to those mediated by alpha-beta TCR.

TCR delta has been associated with certain cancers and infectious diseases including tuberculosis. Gamma delta T cells with TCR delta exhibit cytotoxic activities against tumor cells and their infiltration can correlate with prognosis in cancer patients. Additionally the role of TCR delta in defense against infections involves interaction with other proteins such as heat shock proteins which are commonly expressed by infected cells. The activity of TCR delta in these contexts highlights its importance in immune regulation and potential as a target in therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constant region of T cell receptor (TR) delta chain that participates in the antigen recognition (PubMed : 24600447). Gamma-delta TRs recognize a variety of self and foreign non-peptide antigens frequently expressed at the epithelial boundaries between the host and external environment, including endogenous lipids presented by MH-like protein CD1D and phosphoantigens presented by butyrophilin-like molecule BTN3A1. Upon antigen recognition induces rapid, innate-like immune responses involved in pathogen clearance and tissue repair (PubMed : 23348415, PubMed : 28920588). Binding of gamma-delta TR complex to antigen triggers phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in the CD3 chains by the LCK and FYN kinases, allowing the recruitment, phosphorylation, and activation of ZAP70 that facilitates phosphorylation of the scaffolding proteins LCP2 and LAT. This lead to the formation of a supramolecular signalosome that recruits the phospholipase PLCG1, resulting in calcium mobilization and ERK activation, ultimately leading to T cell expansion and differentiation into effector cells (PubMed : 25674089). Gamma-delta TRs are produced through somatic rearrangement of a limited repertoire of variable (V), diversity (D), and joining (J) genes. The potential diversity of gamma-delta TRs is conferred by the unique ability to rearrange (D) genes in tandem and to utilize all three reading frames. The combinatorial diversity is considerably increased by the sequence exonuclease trimming and random nucleotide (N) region additions which occur during the V-(D)-J rearrangements (PubMed : 24387714).
See full target information TRDC
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com