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Rabbit Recombinant Monoclonal TLE 1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TLE 1 antibody [EPR9386(2)] (AB198525), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species
Human
Dilution info
1/100
Notes

This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

2 products for Alternative Version

Target data

Function

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Enhances FOXG1/BF-1- and HES1-mediated transcriptional repression (By similarity). The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

Alternative names

Transducin-like enhancer protein 1, E(Sp1) homolog, Enhancer of split groucho-like protein 1, ESG1, TLE1

Recommended products

Rabbit Recombinant Monoclonal TLE 1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9386(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Transducin-Like Enhancer of Split 1 also known as TLE1 is a transcriptional corepressor well-known for its involvement in Notch and Wnt signaling pathways. TLE1 has a molecular mass of approximately 77 kDa. This protein is expressed in various tissues including the brain heart and lung highlighting its involvement in multiple biological systems. Researchers recognize its importance in modulating gene expression through interactions with different transcription factors.

Biological function summary

The TLE1 protein plays a role in embryonic development by regulating the transcription of genes critical for cell differentiation. TLE1 forms part of a complex with other proteins to exert its corepressive function effectively. Through these interactions TLE1 is involved in differentiation processes in cells such as MCF-7 a breast cancer cell line influencing cellular behavior and function.

Pathways

TLE1 influences the Notch and Wnt signaling pathways essential for cell fate determination and proliferation. Its interaction with key proteins like β-catenin and Notch receptor proteins connects it to these pathways modulating the transcription of target genes. TLE1's role in these pathways is important for maintaining balanced cellular processes and preventing abnormal cell growth.

Associated diseases and disorders

TLE1 is linked to various forms of cancer including synovial sarcoma and breast cancer. Its overexpression has been observed in these cancers often in conjunction with other proteins like BCL2 supporting cell survival and resisting apoptosis. TLE1's involvement in these diseases makes it a target for therapeutic strategies aimed at modulating its activity to inhibit tumor progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TLE 1 antibody [EPR9386(2)] (ab198525), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TLE 1 antibody [EPR9386(2)] (ab198525)

    ab198525 staining TLE 1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198525 at 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5min).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com