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AB225494

Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] - Microglial marker

3

(2 Reviews)

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(9 Publications)

Anti-TMEM119 antibody [106-6] - Alexa Fluor® 647 conjugated (ab225494) is a rabbit recombinant monoclonal antibody detecting TMEM119 in Flow Cytometry (Intra), Flow Cytometry. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Transmembrane protein 119, Osteoblast induction factor, OBIF, Tmem119

1 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] - Microglial marker (AB225494)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] - Microglial marker (AB225494)

Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225494 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor® 647 ab199093 (left). Cells were surface-stained with ab225494, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 488 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

  • 578 PE

    PE Anti-TMEM119 antibody [106-6] - Microglial marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker

  • Unconjugated

    Anti-TMEM119 antibody [106-6] - Microglial marker

  • Carrier free

    Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

106-6

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2026, QC testing of replenishment batches of this primary conjugate changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, the replenishment lots are subjected to bioanalytical testing only. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p><strong>The cellular localisation of this product has been verified in ICC/IF.</strong></p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] (ab225494) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.

Related products
Antibody clone 106-6 is also available pre-conjugated to a variety of labels for your convenience – Anti-TMEM119 Alexa Fluor® 647 [106-6] (ab225494).

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM119 also known as transmembrane protein 119 plays a role in cell membrane dynamics. It has a molecular mass of approximately 35 kDa. TMEM119 is specifically expressed in microglia in the central nervous system. Microglia serve essential functions in brain homeostasis and immune response. Given its expression profile TMEM119 serves as a marker to identify microglia especially in research focused on neurological tissues. Researchers often utilize techniques like microglial flow cytometry and microglial staining for identification and quantification of microglial populations often in frozen marker studies.
Biological function summary

TMEM119 regulates signaling pathways that maintain microglial identity and function but does not typically form part of larger complexes. It modulates microglial activation states and influences responses to central nervous system injury and inflammation. As a membrane protein it may interact with other surface molecules although specific binding partners are not well-characterized in current literature. Researchers value TMEM119 as a reliable marker in studies aiming to distinguish brain-resident microglia from infiltrating peripheral macrophages.

Pathways

TMEM119 functions as a modulator within inflammatory and neuroimmune pathways. It plays roles in the NF-kB signaling and the neuroinflammatory cascade. Its relationship with proteins like CD68 and Iba1 indicates it may influence pathways tied to immune responses and phagocytosis within nervous system contexts. Understanding the interactions and co-expression of TMEM119 with these proteins helps clarify its contribution to neuroimmune functions.

TMEM119 links with neurological conditions such as Alzheimer's disease and multiple sclerosis. Altered expression of TMEM119 appears in disease states potentially contributing to pathogenesis through disrupted inflammatory responses. Its association with proteins such as TREM2 in Alzheimer's suggests that TMEM119 may play indirect roles in the progression of neurodegeneration. Furthermore high TMEM119 levels in pathological samples provide insights into microglial involvement in disease progression and potential targets for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in bone formation and normal bone mineralization (PubMed : 20025746, PubMed : 22416756, PubMed : 26207632). Promotes the differentiation of myoblasts into osteoblasts (PubMed : 20025746, PubMed : 22416756, PubMed : 22579779). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway (PubMed : 21239498, PubMed : 22579779). Up-regulates the expression of ATF4 which plays a central role in osteoblast differentiation (PubMed : 24362451). Essential for normal spermatogenesis and late testicular differentiation (PubMed : 26207632).
See full target information Tmem119
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eado9751 PubMed39919185

2025

Inflammatory responses to acute carbon monoxide poisoning and the role of plasma gelsolin.

Applications

Unspecified application

Species

Unspecified reactive species

Awadhesh K Arya,Kinjal Sethuraman,Jaylyn Waddell,Yong Sung Cha,Yuanyuan Liang,Veena M Bhopale,Abid R Bhat,Zuha Imtiyaz,Alik Dakessian,Yoonsuk Lee,Stephen R Thom

European journal of medical research 30:80 PubMed39910594

2025

Olfactory mucosa-mesenchymal stem cells with overexpressed Nrf2 modulate angiogenesis and exert anti-inflammation effect in an in vitro traumatic brain injury model.

Applications

Unspecified application

Species

Unspecified reactive species

Zigui Chen,Chunyuan Zhang,Yuhua Fang,He Zhang,Jiawei Luo,Changfeng Miao,Jiale Li,Jun Peng,Yingqi Qiu,Ying Xia,Qisheng Luo

Physiological reports 12:e70130 PubMed39567182

2024

Influence of exposure duration on human pressure-induced inflammatory responses: Comparison between tunnel workers and underwater divers.

Applications

Unspecified application

Species

Unspecified reactive species

Zuha Imtiyaz,Owen J O'Neill,Douglas Sward,Phi-Nga Jeannie Le,Awadhesh K Arya,Veena M Bhopale,Abid R Bhat,Stephen R Thom

Diagnostics (Basel, Switzerland) 14: PubMed38928708

2024

Microparticles in Human Perspiration as an Inflammatory Response Index.

Applications

Unspecified application

Species

Unspecified reactive species

Zuha Imtiyaz,Veena M Bhopale,Awadhesh K Arya,Abid R Bhat,Stephen R Thom

Journal of applied physiology (Bethesda, Md. : 1985) 137:63-73 PubMed38660728

2024

Persistent neuroinflammation and functional deficits in a murine model of decompression sickness.

Applications

Unspecified application

Species

Unspecified reactive species

Abid R Bhat,Awadhesh K Arya,Veena M Bhopale,Zuha Imtiyaz,Su Xu,Dilara Bedir,Stephen R Thom

Technology and health care : official journal of the European Society for Engineering and Medicine 31:409-421 PubMed37066940

2023

Acupuncture improves learning and memory ability of posttraumatic stress disorder model rats through epigenetic regulation of microglial phosphatidylinositol 3-kinase pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mi Li,Xian Wang,Lijie Yang,Yong Jiang,Yiqiang Xie,Kai Li

Journal of neurophysiology 129:662-671 PubMed36752495

2023

Neuroinflammation with increased glymphatic flow in a murine model of decompression sickness.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen R Thom,Veena M Bhopale,Abid R Bhat,Awadhesh K Arya,Deepa Ruhela,Guanda Qiao,Xin Li,Shiyu Tang,Su Xu

Iranian journal of basic medical sciences 26:30-36 PubMed36594068

2023

LSD1 in beige adipocytes protects cardiomyocytes against oxygen and glucose deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqiu Cao,Zhu Dong,Dongpeng Yang,Xiaowu Wang

Brain, behavior, & immunity - health 18:100398 PubMed34917988

2021

Astrocyte-derived microparticles initiate a neuroinflammatory cycle due to carbon monoxide poisoning.

Applications

Unspecified application

Species

Unspecified reactive species

Deepa Ruhela,Veena M Bhopale,Sudhakar Kalakonda,Stephen R Thom
View all publications
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Product promise

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