AB205487

Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker

Name: Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] (ab205487) | Abcam
Brand: Abcam Limited
SKU: AB205487
Price: 630 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(4 Publications)

Rabbit Recombinant Monoclonal TOMM20 antibody - conjugated to Alexa Fluor® 647. Mitochondrion marker. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

KIAA0016, TOMM20, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB205487)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB205487)

ab205487 staining TOMM20 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205487 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
HRP

HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
Unconjugated

Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
Carrier free

Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15581-39

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Central receptor component of the translocase of the outer membrane of mitochondria (TOM) complex essential for the recognition and translocation of cytosolically synthesized mitochondrial preproteins (PubMed : 40080546). Together with TOMM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (PubMed : 18331822). The TOM complex associates with the ion channel VDAC2 and PINK1 kinase at depolarized mitochondria, this interaction stabilizes PINK1 at the outer mitochondrial membrane and triggers downstream mitophagy by the recruitment of the E3 ubiquitin ligase PRKN (PubMed : 40080546). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (By similarity).

See full target information TOMM20

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29167 PubMed40783579

2025

Therapeutic potential of human mesenchymal stromal cell-derived mitochondria in a rat model of surgical digestive fistula.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Mariani,Augustin Guichard,Anna C Sebbagh,André Cronemberger Andrade,Zahra Al Amir Dache,Christopher Ribes,Dmitry Ayollo,Mehdi Karoui,Gregory Lavieu,Florence Gazeau,Amanda K A Silva,Gabriel Rahmi,Sabah Mozafari

Cell metabolism 35:821-836.e7 PubMed36948185

2023

Epigenetic dosage identifies two major and functionally distinct β cell subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Erez Dror,Luca Fagnocchi,Vanessa Wegert,Stefanos Apostle,Brooke Grimaldi,Tim Gruber,Ilaria Panzeri,Steffen Heyne,Kira Daniela Höffler,Victor Kreiner,Reagan Ching,Tess Tsai-Hsiu Lu,Ayush Semwal,Ben Johnson,Parijat Senapati,Adelheid Lempradl,Dustin Schones,Axel Imhof,Hui Shen,John Andrew Pospisilik

Science advances 8:eabo4271 PubMed35704572

2022

CD38 reduces mitochondrial fitness and cytotoxic T cell response against viral infection in lupus patients by suppressing mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ping-Min Chen,Eri Katsuyama,Abhigyan Satyam,Hao Li,Jose Rubio,Sungwook Jung,Sylvia Andrzejewski,J David Becherer,Maria G Tsokos,Reza Abdi,George C Tsokos

Advanced science (Weinheim, Baden-Wurttemberg, Ger 6:1901673 PubMed31763149

2019

Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Eol Park,Dongkil Choi,Ji Su Park,Changgon Sim,Sohyun Park,Sunah Kang,Hyunsoo Yim,Myungsun Lee,Jaeyoun Kim,Jinyoung Pac,Kunsoo Rhee,Junho Lee,Yunjong Lee,Yan Lee,Sung-Yon Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB205487)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise