AB209606

Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

Name: Alexa Fluor® 647 conjugated Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab209606) | Abcam
Brand: Abcam Limited
SKU: AB209606
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(25 Publications)

Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker - Alexa Fluor® 647 conjugated (ab209606) is a rabbit recombinant monoclonal antibody detecting TOMM20 in ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

KIAA0016, TOMM20, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB209606)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB209606)

ab209606 staining TOMM20 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209606 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

Carrier free

Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free
Unconjugated

Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker
660 APC

APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker
HRP

HRP Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker
578 PE

PE Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15581-54

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab209606) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-TOMM20 [EPR15581-54] - Mitochondrial Marker Alexa Fluor® 647 (ab209606) was first used in a scientific publication in 2016 and has been cited over 10 times in peer-reviewed journals.

Related products
Antibody clone EPR15581-54 is also available pre-conjugated to a variety of labels for your convenience – Anti-TOMM20 Alexa Fluor® 647 [EPR15581-54] - Mitochondrial Marker (ab209606).

Other related products
We have a range of other formats of antibody clone [EPR15581-54] also available for your convenience: ab186735, Alexa Fluor® 647 - ab209606, HRP - ab209951, Alexa Fluor® 405 - ab210047, Alexa Fluor® 594 - ab210665, Alexa Fluor® 568 - ab210841, Alexa Fluor® 555 - ab221292, APC - ab225341, PE - ab225342, Carrier free - ab232589, Alexa Fluor® 488 - ab320072

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Central receptor component of the translocase of the outer membrane of mitochondria (TOM) complex essential for the recognition and translocation of cytosolically synthesized mitochondrial preproteins (PubMed : 40080546). Together with TOMM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (PubMed : 18331822). The TOM complex associates with the ion channel VDAC2 and PINK1 kinase at depolarized mitochondria, this interaction stabilizes PINK1 at the outer mitochondrial membrane and triggers downstream mitophagy by the recruitment of the E3 ubiquitin ligase PRKN (PubMed : 40080546). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (By similarity).

See full target information TOMM20

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40806782

2025

The Loss of Complex I in Renal Oncocytoma Is Associated with Defective Mitophagy Due to Lysosomal Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Lin,Neal Patel,Lucia Fernandez-Del-Rio,Cristiane Benica,Blake Wilde,Eirini Christodoulou,Shinji Ohtake,Anhyo Jeong,Aboubacar Kaba,Nedas Matulionis,Randy Caliliw,Xiaowu Gai,Heather Christofk,David Shackelford,Brian Shuch

Nature communications 15:8802 PubMed39438460

2024

In situ visualization of endothelial cell-derived extracellular vesicle formation in steady state and malignant conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Georgia K Atkin-Smith,Jascinta P Santavanond,Amanda Light,Joel S Rimes,Andre L Samson,Jeremy Er,Joy Liu,Darryl N Johnson,Mélanie Le Page,Pradeep Rajasekhar,Raymond K H Yip,Niall D Geoghegan,Kelly L Rogers,Catherine Chang,Vanessa L Bryant,Mai Margetts,M Cristina Keightley,Trevor J Kilpatrick,Michele D Binder,Sharon Tran,Erinna F Lee,Walter D Fairlie,Dilara C Ozkocak,Andrew H Wei,Edwin D Hawkins,Ivan K H Poon

Nature methods 21:1082-1093 PubMed38831208

2024

Universal inverse modeling of point spread functions for SMLM localization and microscope characterization.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Liu,Jianwei Chen,Jonas Hellgoth,Lucas-Raphael Müller,Boris Ferdman,Christian Karras,Dafei Xiao,Keith A Lidke,Rainer Heintzmann,Yoav Shechtman,Yiming Li,Jonas Ries

Science advances 10:eadj3656 PubMed38457497

2024

Large-FOV 3D localization microscopy by spatially variant point spread function generation.

Applications

Unspecified application

Species

Unspecified reactive species

Dafei Xiao,Reut Kedem Orange,Nadav Opatovski,Amit Parizat,Elias Nehme,Onit Alalouf,Yoav Shechtman

Cell reports 42:113260 PubMed37851575

2023

A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher S Waters,Sigurd B Angenent,Steven J Altschuler,Lani F Wu

Light, science & applications 12:222 PubMed37696792

2023

Near index matching enables solid diffractive optical element fabrication via additive manufacturing.

Applications

Unspecified application

Species

Unspecified reactive species

Reut Orange Kedem,Nadav Opatovski,Dafei Xiao,Boris Ferdman,Onit Alalouf,Sushanta Kumar Pal,Ziyun Wang,Henrik von der Emde,Michael Weber,Steffen J Sahl,Aleks Ponjavic,Ady Arie,Stefan W Hell,Yoav Shechtman

JCI insight 8: PubMed37166984

2023

T cell metabolic reprogramming in acute kidney injury and protection by glutamine blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Kyungho Lee,Elizabeth A Thompson,Sepideh Gharaie,Chirag H Patel,Johanna T Kurzhagen,Phillip M Pierorazio,Lois J Arend,Ajit G Thomas,Sanjeev Noel,Barbara S Slusher,Hamid Rabb

Bio-protocol 13: PubMed36816992

2023

High-throughput Assessment of Mitochondrial Protein Synthesis in Mammalian Cells Using Mito-FUNCAT FACS.

Applications

Unspecified application

Species

Unspecified reactive species

Hironori Saito,Tatsuya Osaki,Yoshiho Ikeuchi,Shintaro Iwasaki

Molecular metabolism 64:101566 PubMed35940556

2022

The mitochondrial fission protein Drp1 in liver is required to mitigate NASH and prevents the activation of the mitochondrial ISR.

Applications

Unspecified application

Species

Unspecified reactive species

Janos Steffen,Jennifer Ngo,Sheng-Ping Wang,Kevin Williams,Henning F Kramer,George Ho,Carlos Rodriguez,Krishna Yekkala,Chidozie Amuzie,Russell Bialecki,Lisa Norquay,Andrea R Nawrocki,Mark Erion,Alessandro Pocai,Orian S Shirihai,Marc Liesa

eLife 11: PubMed35852853

2022

Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation.

Applications

Unspecified application

Species

Unspecified reactive species

Cansu Küey,Méghane Sittewelle,Gabrielle Larocque,Miguel Hernández-González,Stephen J Royle

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB209606)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (25)

Product promise