Rabbit Recombinant Monoclonal TOMM20 antibody - conjugated to Alexa Fluor® 647. Mitochondrion marker. Suitable for ICC/IF and reacts with Human samples. Cited in 19 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB209606), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-tomm20-antibody-epr15581-54-mitochondrial-marker-ab209606--immunocytochemistry-immunofluorescence-img68737.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB209606)")
Publications
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- JCI insight 8:2023T cell metabolic reprogramming in acute kidney injury and protection by glutamine blockade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyungho Lee,Elizabeth A Thompson,Sepideh Gharaie,Chirag H Patel,Johanna T Kurzhagen,Phillip M Pierorazio,Lois J Arend,Ajit G Thomas,Sanjeev Noel,Barbara S Slusher,Hamid RabbPubMed 37166984
- Bio-protocol 13:2023High-throughput Assessment of Mitochondrial Protein Synthesis in Mammalian Cells Using Mito-FUNCAT FACS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHironori Saito,Tatsuya Osaki,Yoshiho Ikeuchi,Shintaro IwasakiPubMed 36816992
- Molecular metabolism 64:1015662022The mitochondrial fission protein Drp1 in liver is required to mitigate NASH and prevents the activation of the mitochondrial ISR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJanos Steffen,Jennifer Ngo,Sheng-Ping Wang,Kevin Williams,Henning F Kramer,George Ho,Carlos Rodriguez,Krishna Yekkala,Chidozie Amuzie,Russell Bialecki,Lisa Norquay,Andrea R Nawrocki,Mark Erion,Alessandro Pocai,Orian S Shirihai,Marc LiesaPubMed 35940556
- eLife 11:2022Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCansu Küey,Méghane Sittewelle,Gabrielle Larocque,Miguel Hernández-González,Stephen J RoylePubMed 35852853
- RNA (New York, N.Y.) 28:895-9042022Mito-FUNCAT-FACS reveals cellular heterogeneity in mitochondrial translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYusuke Kimura,Hironori Saito,Tatsuya Osaki,Yasuhiro Ikegami,Taisei Wakigawa,Yoshiho Ikeuchi,Shintaro IwasakiPubMed 35256452
- Molecular cancer therapeutics 20:1469-14802021Therapeutic Synergy in Esophageal Cancer and Mesothelioma Is Predicted by Dynamic BH3 Profiling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeborah R Surman,Yuan Xu,Min-Jung Lee,Jane Trepel,Kate Brown,Maheshwari Ramineni,Taylor G Splawn,Laurence P Diggs,H Courtney Hodges,Jeremy L Davis,Hyun-Sung Lee,Bryan M Burt,Robert Taylor RipleyPubMed 34088830
- Nature communications 12:306720213D printable diffractive optical elements by liquid immersion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesReut Orange-Kedem,Elias Nehme,Lucien E Weiss,Boris Ferdman,Onit Alalouf,Nadav Opatovski,Yoav ShechtmanPubMed 34031389
- PLoS biology 19:e30011662021Neural stem cells traffic functional mitochondria via extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuca Peruzzotti-Jametti,Joshua D Bernstock,Cory M Willis,Giulia Manferrari,Rebecca Rogall,Erika Fernandez-Vizarra,James C Williamson,Alice Braga,Aletta van den Bosch,Tommaso Leonardi,Grzegorz Krzak,Ágnes Kittel,Cristiane Benincá,Nunzio Vicario,Sisareuth Tan,Carlos Bastos,Iacopo Bicci,Nunzio Iraci,Jayden A Smith,Ben Peacock,Karin H Muller,Paul J Lehner,Edit Iren Buzas,Nuno Faria,Massimo Zeviani,Christian Frezza,Alain Brisson,Nicholas J Matheson,Carlo Viscomi,Stefano PluchinoPubMed 33826607
- Cell 183:636-649.e182020TDP-43 Triggers Mitochondrial DNA Release via mPTP to Activate cGAS/STING in ALS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChien-Hsiung Yu et. al.PubMed 33031745
- The Journal of biological chemistry 295:13981-139932020Generation and validation of recombinant antibodies to study human aminoacyl-tRNA synthetases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharlotta Preger et. al.PubMed 32817337
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
8 products for Alternative Version
Target data
Function
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Alternative names
Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOMM20, KIAA0016
Recommended products
Rabbit Recombinant Monoclonal TOMM20 antibody - conjugated to Alexa Fluor® 647. Mitochondrion marker. Suitable for ICC/IF and reacts with Human samples. Cited in 19 publications.
Alternative names
Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOMM20, KIAA0016
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR15581-54
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab209606), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-tomm20-antibody-epr15581-54-mitochondrial-marker-ab209606--immunocytochemistry-immunofluorescence-img68737.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab209606)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab209606)
ab209606 staining TOMM20 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209606 at 1/1000 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).
Downloads
Product protocols
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- Visit troubleshooting
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