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AB200993

Alexa Fluor® 647 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

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(1 Publication)

Rabbit Recombinant Monoclonal Topoisomerase II alpha antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TOP2, TOP2A, DNA topoisomerase 2-alpha

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377] (AB200993)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377] (AB200993)

ab200933 staining Topoisomerase II alpha + Topoisomerase II beta in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200933 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2μg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377] (AB200993)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377] (AB200993)

Overlay histogram showing HeLa cells stained with ab200993 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab200993, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

  • 578 PE

    PE Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377]

  • 660 APC

    APC Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377]

  • HRP

    HRP Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

  • Unconjugated

    Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

  • Carrier free

    Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5377

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Topoisomerase II alpha and Topoisomerase II beta also known as TOP2A and TOP2B are enzymes with a vital role in modulating the topological states of DNA. They actively introduce transient double-strand breaks in the DNA helix to manage DNA tangling and supercoiling during replication and transcription. TOP2A has a molecular weight of approximately 170 kilodaltons while TOP2B weighs around 180 kilodaltons. High levels of TOP2A are observed in proliferating cells such as those in the thymus and testis while TOP2B is expressed in nearly all tissues indicating its ubiquitous nature.
Biological function summary

These enzymes participate in fundamental cellular processes including chromosomal segregation replication and transcription. They function in the context of large protein complexes rather than in isolation. TOP2A is often engaged during mitosis due to its cell cycle-dependent expression. In contrast TOP2B's functions spread across various DNA-related actions such as differentiation and development. This distribution emphasizes their respective regulatory roles in both proliferating and resting cells.

Pathways

TOP2A and TOP2B integrate into critical pathways like the DNA replication and repair pathways. As a notable component of these pathways TOP2A interacts closely with proteins like MCM2-7 and ORC complex for replication origin unwinding while TOP2B shares interactions with factors like RNA polymerase II for transcription-related activities. These interactions reflect their involvement in maintaining genomic stability across different cellular conditions.

Aberrations in TOP2A expression or function link closely to numerous cancers reflecting its role in cell proliferation. Furthermore mutations or dysregulation of TOP2B have implications in disorders related to neural development highlighting its role beyond cellular growth control. Other related proteins like BRCA1 also display interactions with TOP2A reinforcing the enzyme's involvement in cancer-related pathways whereas TOP2B’s association with proteins like TAF(II)250 suggests a broader involvement in transcription processes relevant to neural disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed : 17567603, PubMed : 18790802, PubMed : 22013166, PubMed : 22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).
See full target information TOP2A

Additional targets

DNA topoisomerase 2-beta
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 293:9662-9673 PubMed29610274

2018

The effectors SseF and SseG inhibit Rab1A-mediated autophagy to facilitate intracellular bacterial survival and replication.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao-Zhong Feng,An-Jie Jiang,An-Wen Mao,Yuhan Feng,Weinan Wang,Jingjing Li,Xiaoyan Zhang,Ke Xing,Xue Peng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com