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AB204011

Alexa Fluor® 647 Anti-TPPP antibody [EPR3316]

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(1 Publication)

Rabbit Recombinant Monoclonal TPPP antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TPPP1, TPPP, Tubulin polymerization-promoting protein, 25 kDa brain-specific protein, TPPP/p25, p24, p25-alpha

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TPPP antibody [EPR3316] (AB204011)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TPPP antibody [EPR3316] (AB204011)

ab204011 staining TPPP in SKNSH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204011 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in SKNSH cells fixed with 100% methanol (5 min).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3316

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TPPP also known as Tubulin Polymerization Promoting Protein or p25 is a small protein with an approximate mass of 25 kDa. TPPP is expressed mainly in the brain particularly abundant in oligodendrocytes. This protein plays an important role in regulating the polymerization of tubulin into microtubules. Through its binding to tubulin TPPP stabilizes microtubule structures facilitating proper microtubule assembly and maintenance. The modulation of these structures is essential for cellular operations like intracellular transport and cell division.
Biological function summary

The protein participates in the dynamics of cytoskeletal elements critical for maintaining cell shape and function. TPPP is part of cytoskeletal networks where it contributes to cellular integrity in neurons. TPPP's activity influences the formation and maintenance of myelin the insulating layer enveloping nerve fibers which is important for fast and efficient nerve impulse conduction. In its role TPPP interacts cooperatively with other cytoskeletal proteins enhancing microtubule resilience and cell structural stability.

Pathways

TPPP interacts in processes involving microtubule organization and stabilization. It is integral in the MAPK/ERK signaling pathway which is prominent in regulating cellular processes like proliferation and differentiation. Additionally TPPP may interplay with proteins such as MAP2 and Tau both involved in the MAP-kinase pathways regulating microtubule dynamics indicating a collaborative network in cellular signaling and structural reinforcement in neural cells.

TPPP has correlations with neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. Abnormal regulation or aggregation of TPPP is linked to the formation of pathological protein inclusions found in these conditions. The interaction between TPPP and Alpha-synuclein is particularly notable as TPPP may regulate or enhance the aggregation of Alpha-synuclein contributing to the development of Lewy bodies in Parkinson's. Understanding these interactions and pathways is essential for developing therapeutic strategies targeting these neurodegenerative disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulator of microtubule dynamics that plays a key role in myelination by promoting elongation of the myelin sheath (PubMed : 31522887). Acts as a microtubule nucleation factor in oligodendrocytes : specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, and promotes microtubule nucleation, an important step for elongation of the myelin sheath (PubMed : 31522887, PubMed : 33831707). Required for both uniform polarized growth of distal microtubules as well as directing the branching of proximal processes (PubMed : 31522887). Shows magnesium-dependent GTPase activity; the role of the GTPase activity is unclear (PubMed : 21316364, PubMed : 21995432). In addition to microtubule nucleation activity, also involved in microtubule bundling and stabilization of existing microtubules, thereby maintaining the integrity of the microtubule network (PubMed : 17105200, PubMed : 17693641, PubMed : 18028908, PubMed : 26289831). Regulates microtubule dynamics by promoting tubulin acetylation : acts by inhibiting the tubulin deacetylase activity of HDAC6 (PubMed : 20308065, PubMed : 23093407). Also regulates cell migration : phosphorylation by ROCK1 inhibits interaction with HDAC6, resulting in decreased acetylation of tubulin and increased cell motility (PubMed : 23093407). Plays a role in cell proliferation by regulating the G1/S-phase transition (PubMed : 23355470). Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis; this process is regulated by phosphorylation by LIMK2 (PubMed : 22328514).
See full target information TPPP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:4354 PubMed34272374

2021

SARS-CoV-2 infection induces the dedifferentiation of multiciliated cells and impairs mucociliary clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Rémy Robinot,Mathieu Hubert,Guilherme Dias de Melo,Françoise Lazarini,Timothée Bruel,Nikaïa Smith,Sylvain Levallois,Florence Larrous,Julien Fernandes,Stacy Gellenoncourt,Stéphane Rigaud,Olivier Gorgette,Catherine Thouvenot,Céline Trébeau,Adeline Mallet,Guillaume Duménil,Samy Gobaa,Raphaël Etournay,Pierre-Marie Lledo,Marc Lecuit,Hervé Bourhy,Darragh Duffy,Vincent Michel,Olivier Schwartz,Lisa A Chakrabarti
View all publications
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Product promise

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