JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB225219

Alexa Fluor® 647 Anti-TTC11/FIS1 antibody [EPR8412]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal TTC11/FIS1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TTC11, CGI-135, FIS1, Mitochondrial fission 1 protein, FIS1 homolog, Tetratricopeptide repeat protein 11, hFis1, TPR repeat protein 11

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TTC11/FIS1 antibody [EPR8412] (AB225219)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TTC11/FIS1 antibody [EPR8412] (AB225219)

ab225219 staining TTC11/FIS1 in Ramos cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225219 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in Ramos cells fixed with 80% methanol (5 min).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8412

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in Ramos cells fixed with 4% formaldehyde (10 min) and 80% methanol (5 min)</p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTC11 also known as FIS1 is a protein that plays an important role in mitochondrial dynamics. It has a molecular weight of approximately 17 kDa. The FIS1 protein is expressed in various tissues suggesting it has a broad functional impact across different cell types. Through its structure it facilitates mitochondrial fission by interacting with other mitochondrial proteins. This process ensures proper distribution and health of mitochondria within the cell.
Biological function summary

FIS1 is critical in controlling mitochondrial size and quantity. It is part of a complex that includes proteins like DRP1 which is heavily involved in promoting the division of mitochondria. By working with DRP1 FIS1 helps maintain cellular energy levels and dynamics by ensuring that mitochondria are appropriately divided and distributed. This role impacts overall cell health and function.

Pathways

FIS1 is integral to the pathways regulating mitochondrial fission and fusion. It interacts closely within the pathway involving DRP1 and MFF both of which are critical in these processes. Furthermore FIS1's interactions in these pathways help balance mitochondrial fission and fusion maintaining cellular energy homeostasis and proper mitochondrial function.

Defects in FIS1 function relate to neurodegenerative diseases such as Alzheimer's disease and Charcot-Marie-Tooth disease. In these contexts abnormal interactions with DRP1 can lead to impaired mitochondrial dynamics contributing to disease progression. Research into these relationships continues to uncover the important role FIS1 plays in maintaining cellular health and its potential as a therapeutic target.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the fragmentation of the mitochondrial network and its perinuclear clustering (PubMed : 12783892, PubMed : 12861026, PubMed : 14996942, PubMed : 23283981). Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission (PubMed : 12861026, PubMed : 16118244, PubMed : 23283981, PubMed : 23530241, PubMed : 24196833). May not be essential for the assembly of functional fission complexes and the subsequent membrane scission event (PubMed : 23530241, PubMed : 24196833). Also mediates peroxisomal fission (PubMed : 16107562). May act when the products of fission are directed toward mitochondrial homeostasis, mitophagy, or apoptosis (PubMed : 24196833). Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis (PubMed : 12783892).
See full target information FIS1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 23:1256-1272 PubMed35902638

2022

Partial RAG deficiency in humans induces dysregulated peripheral lymphocyte development and humoral tolerance defect with accumulation of T-bet B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Krisztian Csomos,Boglarka Ujhazi,Peter Blazso,Jose L Herrera,Christopher M Tipton,Tomoki Kawai,Sumai Gordon,Maryssa Ellison,Kevin Wu,Matthew Stowell,Lauren Haynes,Rachel Cruz,Bence Zakota,Johnny Nguyen,Michelle Altrich,Christoph B Geier,Svetlana Sharapova,Joseph F Dasso,Jennifer W Leiding,Grace Smith,Waleed Al-Herz,Mayra de Barros Dorna,Olajumoke Fadugba,Eva Fronkova,Veronika Kanderova,Michael Svaton,Sarah E Henrickson,Joseph D Hernandez,Taco Kuijpers,Snezhina Mihailova Kandilarova,Elizaveta Naumova,Tomas Milota,Anna Sediva,Despina Moshous,Benedicte Neven,Tara Saco,Ravishankar Sargur,Sinisa Savic,John Sleasman,Gauri Sunkersett,Brant R Ward,Masanobu Komatsu,Stefania Pittaluga,Attila Kumanovics,Manish J Butte,Michael P Cancro,Shiv Pillai,Eric Meffre,Luigi D Notarangelo,Jolan E Walter
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB96764

Anti-TTC11/FIS1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TTC11/FIS1 antibody (AB96764)

  • 4
  • 2
Primary Antibodies

AB189846

Anti-TTC11/FIS1 antibody - N-terminal

Western blot - Anti-TTC11/FIS1 antibody - N-terminal (AB189846)

  • 0
  • 0
Primary Antibodies

AB71498

Anti-TTC11/FIS1 antibody

Western blot - Anti-TTC11/FIS1 antibody (AB71498)

  • 4
  • 3
Primary Antibodies

AB229969

Anti-TTC11/FIS1 antibody

Western blot - Anti-TTC11/FIS1 antibody (AB229969)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB184247

Anti-DRP1 antibody [EPR19274]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-DRP1 antibody [EPR19274] (AB184247)

  • 5
  • 5
Primary Antibodies

AB126575

Anti-Mitofusin 1 antibody [11E91H12]

Western blot - Anti-Mitofusin 1 antibody [11E91H12] (AB126575)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB272921

Anti-TOMM40 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody (AB272921)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com