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Rat Monoclonal Alpha-tubulin 1 acetyl K40 antibody - conjugated to Alexa Fluor® 647. Microtubule marker. Suitable for ICC/IF and reacts with Human, Alligator samples. Cited in 4 publications.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195884), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195884), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195884), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195884), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195884), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Rat
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Predicted
Rat
Predicted
Alligator
Tested
Dog
Predicted
Saccharomyces cerevisiae
Predicted
Schizosaccharomyces pombe
Predicted

Tested
Tested

Species
Human, Alligator
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Dog, Saccharomyces cerevisiae, Schizosaccharomyces pombe
Dilution info
-
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

2 products for Alternative Version

Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871).

Alternative names

Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B

Recommended products

Rat Monoclonal Alpha-tubulin 1 acetyl K40 antibody - conjugated to Alexa Fluor® 647. Microtubule marker. Suitable for ICC/IF and reacts with Human, Alligator samples. Cited in 4 publications.

Key facts

Isotype
IgG2a
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
YOL1/34
Purity
IgG fraction
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tubulin sometimes called DM1B is a globular protein essential for the formation of microtubules. It has a molecular mass of approximately 55 kDa. Tubulin is expressed in most eukaryotic cells and exists as two closely related subunits alpha-tubulin and beta-tubulin. These subunits polymerize to form linear microtubule structures. Anti-tubulin antibodies such as anti-tubulin Alexa 34 can detect these microtubule markers in cells allowing researchers to visualize cytoskeletal organization.

Biological function summary

This core component of microtubule assembly is important for maintaining cell shape enabling intracellular transport and segregating chromosomes during cell division. As part of a complex tubulin interacts with various microtubule-associated proteins (MAPs) that regulate its dynamic assembly and disassembly. This regulation is important for processes like axonal transport in neurons and the movement of cilia and flagella in other cell types.

Pathways

Tubulin plays a significant role in the mitotic spindle assembly part of the cell cycle. This process is vital for the accurate segregation of chromosomes to daughter cells. Tubulin interacts with the kinesin and dynein motor proteins within this pathway which are essential for intracellular transport and mitosis. Another key pathway involving tubulin is the intracellular trafficking facilitated by motor proteins which is necessary for maintaining cell homeostasis and function.

Associated diseases and disorders

Aberrant regulation or mutations in tubulin can lead to conditions such as cancer and neurodegenerative diseases. In cancer altered tubulin dynamics contribute to uncontrolled cell proliferation. Neurodegenerative disorders such as Alzheimer’s disease show involvement with tubulin through abnormal microtubule stability often linked to the MAP tau protein. Anti-tubulin antibodies including LAMP1 can help in research related to these conditions by offering methods to study tubulin distribution and function in diseased cells.

Product promise

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7 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)

    ab195884 staining Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195884 at a 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)

    ab195884 staining Tubulin in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195884 at a 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)

    Alexa Fluor® 555 Anti-Cytokeratin 14 antibody [LL002] ab320066 staining Cytokeratin 14 in A-431 cells, with negative expression in KRT14 knockout A-431 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 555 Anti-Cytokeratin 14 antibody [LL002] ab320066 at 0.2 ug/ml (shown in green). ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647) (shown in magenta) was used at 2 ug/ml for structural counterstaining.

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    This product also work with 100% methanol (5 min) fixation under the same testing conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)

    Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab320072 staining TOMM20 in HeLa cells, with negative expression in cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab320072 at 0.2 ug/ml (shown in Green). ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647) (shown in magenta) was used at 2 ug/ml for structural counterstaining.

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    This product also work with 100% methanol (5 min) fixation under the same testing conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling Ubiquitin (phospho S65) with Anti-Ubiquitin (phospho S65) antibody [30H3/30K1] - Rat IgG2a (Chimeric) ab320096 at 1/50 (18.68 ug/ml) dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing increased staining in PC-3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab195884 Anti-Tubulin rat monoclonal antibody - Microtubule Marker (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 2ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)

    Alexa Fluor® 488 Anti-Myogenin antibody [F5D] ab320062 staining myogenin in 48h differenciated-C2C12 cells (2% Horse Serum + 1uM Insulin for 48hr), with negative expression in undifferenciated C2C12 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 488 Anti-Myogenin antibody [F5D] ab320062 at 0.5 ug/ml (shown in green). ab195884, rat monoclonal to Tubulin (Alexa Fluor® 647) (shown in magenta) was used at 2 ug/ml for structural counterstaining.

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    This product also work with 100% methanol (5 min) fixation under the same testing conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)

    Alexa Fluor® 488 Anti-Cytokeratin 14 antibody [LL002] ab320070 staining Cytokeratin14 in A-431 cells, with negative expression in KRT14 knockout A-431 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 488 Anti-Cytokeratin 14 antibody [LL002] ab320070 at 1 ug/ml (shown in green). ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647) (shown in magenta) was used at 2 ug/ml for structural counterstaining.

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    This product also work with 100% methanol (5 min) fixation under the same testing conditions.

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Product protocols

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