Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195884)

ab320070 staining Cytokeratin14 in A-431 cells, with negative expression in KRT14 knockout A-431 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab320070 at 1 ug/ml (shown in green). ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647) (shown in magenta) was used at 2 ug/ml for structural counterstaining.

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

This product also work with 100% methanol (5 min) fixation under the same testing conditions.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195884)

ab195884 staining Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195884 at a 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195884)

ab320066 staining Cytokeratin 14 in A-431 cells, with negative expression in KRT14 knockout A-431 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab320066 at 0.2 ug/ml (shown in green). ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647) (shown in magenta) was used at 2 ug/ml for structural counterstaining.

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

This product also work with 100% methanol (5 min) fixation under the same testing conditions.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195884)

ab195884 staining Tubulin in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195884 at a 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195884)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling Ubiquitin (phospho S65) with ab320096 at 1/50 (18.68 ug/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing increased staining in PC-3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195884 Anti-Tubulin rat monoclonal antibody - Microtubule Marker (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195884)

ab320072 staining TOMM20 in HeLa cells, with negative expression in cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab320072 at 0.2 ug/ml (shown in Green). ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647) (shown in magenta) was used at 2 ug/ml for structural counterstaining.

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

This product also work with 100% methanol (5 min) fixation under the same testing conditions.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195884)

ab320062 staining myogenin in 48h differenciated-C2C12 cells (2% Horse Serum + 1uM Insulin for 48hr), with negative expression in undifferenciated C2C12 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab320062 at 0.5 ug/ml (shown in green). ab195884, rat monoclonal to Tubulin (Alexa Fluor^® 647) (shown in magenta) was used at 2 ug/ml for structural counterstaining.

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

This product also work with 100% methanol (5 min) fixation under the same testing conditions.