Alexa Fluor® 647 Anti-Uteroglobin antibody [EPR27144-86]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Uteroglobin antibody [EPR27144-86] (AB317531)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Uteroglobin with ab317531 at 1/100 (5.0 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a human Uteroglobin expression vector containing a myc-his tag (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Uteroglobin antibody [EPR27144-86] (AB317531)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Uteroglobin with ab317531 at 1/100 (5.0 ug/ml) dilution.

Negative control : no staining on human kidney.The section was incubated with ab317531 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Uteroglobin antibody [EPR27144-86] (AB317531)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Uteroglobin with ab317531 at 1/100 (5.0 ug/ml) dilution.

Positive staining on the airway epithelium of human lung. The section was incubated with ab317531 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins