AB196579

Alexa Fluor® 647 Anti-Vinculin antibody [EPR8185]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal Vinculin antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

Vinculin, Metavinculin, MV, VCL

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Vinculin antibody [EPR8185] (AB196579)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Vinculin antibody [EPR8185] (AB196579)

ab196579 staining Vinculin in HUVEC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196579 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Carrier free

Anti-Vinculin antibody [EPR8185] - BSA and Azide free
Unconjugated

Anti-Vinculin antibody [EPR8185] - Loading Control
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Vinculin antibody [EPR8185]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Vinculin antibody [EPR8185]
HRP

HRP Anti-Vinculin antibody [EPR8185] - Loading Control

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8185

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "We recommend using this antibody with methanol fixation only. Use of paraformaldehyde fixation may result in non-specific signal. Use at a low concentration to minimize background. We have observed the best results in A431 and HeLa cells when using the antibody in the 0.04 µg/mL to 0.008 µg/mL range.", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

See full target information VCL

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4271 PubMed37460553

2023

The checkpoint inhibitor PD-1H/VISTA controls osteoclast-mediated multiple myeloma bone disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Fu,Shirong Li,Huihui Ma,Jun Yang,Gabriel M Pagnotti,Lewis M Brown,Stephen J Weiss,Markus Y Mapara,Suzanne Lentzsch

Bioactive materials 5:1044-1052 PubMed32695935

2020

Insight into vitronectin structural evolution on material surface chemistries: The mediation for cell adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Tianjie Li,Lijing Hao,Jiangyu Li,Chang Du,Yingjun Wang

eLife 8: PubMed31429823

2019

CCN1 interlinks integrin and hippo pathway to autoregulate tip cell activity.

Applications

Unspecified application

Species

Unspecified reactive species

Myo-Hyeon Park,Ae Kyung Kim,Sarala Manandhar,Su-Young Oh,Gun-Hyuk Jang,Li Kang,Dong-Won Lee,Do Young Hyeon,Sun-Hee Lee,Hye Eun Lee,Tae-Lin Huh,Sang Heon Suh,Daehee Hwang,Kyunghee Byun,Hae-Chul Park,You Mie Lee

International journal of oral science 11:18 PubMed31152146

2019

Compliant substratum modulates vinculin expression in focal adhesion plaques in skeletal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chenchen Zhou,Qingxuan Wang,Demao Zhang,Linyi Cai,Wei Du,Jing Xie

ACS applied materials & interfaces 10:26917-26927 PubMed30004214

2018

Microenvironmental Stiffness Regulates Dental Papilla Cell Differentiation: Implications for the Importance of Fibronectin-Paxillin-β-Catenin Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingru Bai,Jing Xie,Xiaoyu Liu,Xia Chen,Wenjing Liu,Fanzi Wu,Dian Chen,Yimin Sun,Xin Li,Chenglin Wang,Ling Ye

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Vinculin antibody [EPR8185]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Vinculin antibody [EPR8185] (AB196579)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (5)

Product promise