Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131]

6 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (ab307377)

  Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Von Willebrand Factor with ab307377 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on endothelial cells of mouse lung.The section was incubated with ab307377 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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  Flow Cytometry - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (ab307377)

  Flow cytometric analysis of Mouse blood cells cells labelling Von Willebrand Factor with ab307377 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor? 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Cells were stained with rabbit IgG or ab307377. Then stained with anti-CD41 conjugated to BV421.Gated on viable cells.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (ab307377)

  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Von Willebrand Factor with ab307377 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on megakaryocytes and platelets of mouse spleen.The section was incubated with ab307377 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (ab307377)

  Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling Von Willebrand Factor with ab307377 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on endothelial cells of rat lung.The section was incubated with ab307377 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (ab307377)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Von Willebrand Factor with ab307377 at 1/500 dilution (1.0 µg/ml). Confocal image showing positive staining on endothelial cells of mouse liver. The nuclear counterstain was DAPI (Blue).

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (ab307377)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Von Willebrand Factor with ab307377 at 1/500 dilution (1.0 µg/ml). Confocal image showing positive staining on endothelial cells of rat liver. The nuclear counterstain was DAPI (Blue).