Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131]

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (AB307377)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (perfused fixed-fresh frozen) tissue, negative control, labeling Glycam1 with ab324912 at 1/500 (1.01 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). ab307377 was used as a co-localization positive control (magenta).

Panel A : merged staining of anti-GLYCAM1 (ab324912, green), anti-VWF (ab307377, magenta) on mouse liver.

Panel B : anti-GLYCAM1 stained on mouse liver.

Panel C : anti-VWF stained in endothelial cells in mouse liver.

The section was incubated in two rounds of staining : in the order of ab324912 and ab307377, for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (AB307377)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lymph node (fresh frozen) tissue labeling Glycam1 with ab324912 at 1/500 (1.01 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). ab307377 was used as a co-localization positive control staining high endothelial venules (magenta).

Panel A : merged staining of anti-GLYCAM1 (ab324912, green), anti-VWF (ab307377, magenta) on mouse lymph node.

Panel B : anti-GLYCAM1 stained on mouse lymph node.

Panel C : anti-VWF stained in high endothelial venules (HEV) of mouse lymph node.

The section was incubated in two rounds of staining : in the order of ab324912 and ab307377, for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (AB307377)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Von Willebrand Factor with ab307377 at 1/500 dilution (1.0 µg/ml). Confocal image showing positive staining on endothelial cells of mouse liver. The nuclear counterstain was DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (AB307377)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Von Willebrand Factor with ab307377 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on endothelial cells of mouse lung.The section was incubated with ab307377 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (AB307377)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling Von Willebrand Factor with ab307377 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on endothelial cells of rat lung.The section was incubated with ab307377 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (AB307377)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Von Willebrand Factor with ab307377 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on megakaryocytes and platelets of mouse spleen.The section was incubated with ab307377 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (AB307377)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Von Willebrand Factor with ab307377 at 1/500 dilution (1.0 µg/ml). Confocal image showing positive staining on endothelial cells of rat liver. The nuclear counterstain was DAPI (Blue).

• Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPR25069-131] (AB307377)

Flow cytometric analysis of Mouse blood cells cells labelling Von Willebrand Factor with ab307377 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor^® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Cells were stained with rabbit IgG or ab307377. Then stained with anti-CD41 conjugated to BV421.Gated on viable cells.