AB195029

Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Von Willebrand Factor antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

F8VWF, VWF, von Willebrand factor, vWF

3 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195029)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195029)

ab195029 staining Von Willebrand Factor in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195029 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195029)

ab195029 staining Von Willebrand Factor in HepG2 (human hepatocellular carcinoma epithelial cell) cells. The cells were fixed with 100% methanol and permeabilised in 0.1% Triton X-100. The cells were then incubated with ab195029 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/200. Nuclear DNA was labelled in blue with DAPI. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195029)

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195029)

Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling Von Willebrand Factor with ab195029 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. ab199093 was used as isotype control (black). Unlabelled control/colour : Cells without incubation with primary antibody and secondary antibody (blue).

Carrier free

Anti-Von Willebrand Factor antibody [EPSISR15] - BSA and Azide free
Unconjugated

Anti-Von Willebrand Factor antibody [EPSISR15]
660 APC

APC Anti-Von Willebrand Factor antibody [EPSISR15]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Von Willebrand Factor antibody [EPSISR15]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Von Willebrand Factor antibody [EPSISR15]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Von Willebrand Factor antibody [EPSISR15]
HRP

HRP Anti-Von Willebrand Factor antibody [EPSISR15]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPSISR15

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human

Applications

Flow Cyt (Intra), Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

See full target information VWF

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 14:e70707 PubMed40028673

2025

Secretomes From Non-Small Cell Lung Cancer Cells Induce Endothelial Plasticity Through a Partial Endothelial-to-Mesenchymal Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Clara Bourreau,Emilie Navarro,Marine Cotinat,Morgane Krejbich,François Guillonneau,Catherine Guette,Alice Boissard,Cécile Henry,Isabelle Corre,Lucas Treps,Nicolas Clere

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195029)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195029)

Flow Cytometry - Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195029)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise