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Rabbit Recombinant Monoclonal WFS1 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr and reacts with Mouse samples.

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Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-WFS1 antibody [EPR23801-91] (AB322307), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-Fr
Mouse
Tested

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Target data

Function

Participates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store (By similarity). Negatively regulates the ER stress response and positively regulates the stability of V-ATPase subunits ATP6V1A and ATP1B1 by preventing their degradation through an unknown proteasome-independent mechanism (By similarity).

Alternative names

Wolframin, Wfs1

Rabbit Recombinant Monoclonal WFS1 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr and reacts with Mouse samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23801-91
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

WFS1 also known as Wolfram Syndrome 1 protein is involved in localizing to the endoplasmic reticulum (ER) membrane. It consists of approximately 890 amino acids and has a molecular mass of about 100 kDa. It is mainly expressed in the brain pancreas and heart reflecting its structural role in cellular organelles. The protein has ER-luminal and cytoplasmic domains which implicate it in maintaining ER function and homeostasis.

Biological function summary

This protein plays a role in modulating ER stress responses and calcium homeostasis. It is a part of a signaling complex that influences apoptotic pathways by regulating cellular stress responses. WFS1 affects the stability and function of the ER having an influence over insulin production and secretion. This regulation is important for cells especially those in the pancreatic islets as it affects the secretion of insulin in response to varying glucose levels.

Pathways

WFS1 integrates into the unfolded protein response (UPR) and calcium signaling pathways. These pathways connect to other proteins like ATF6 and PERK which mediate stress-induced responses and adaptations in cells. In the context of insulin regulation the protein contributes to vesicular fusion mechanisms and secretory pathways that facilitate insulin release. WFS1 influences glucose metabolism through direct interactions in pathways regulating cellular stress and protein folding.

Associated diseases and disorders

Mutations in this gene relate to Wolfram syndrome a condition characterized by diabetes insipidus diabetes mellitus optic atrophy and deafness. These mutations also connect to disorders like ataxia due to their involvement in neurodegenerative processes. WFS1 dysfunction impacts insulin regulation linking it with proteins like MANF that also mitigate ER stress and express protective roles against cellular stress conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-WFS1 antibody [EPR23801-91] (ab322307), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-WFS1 antibody [EPR23801-91] (ab322307)

    Immunofluorescence staining of WFS1 in a section of frozen mouse pancreas.

    The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BONDTM. The section was incubated at room temperature for 1 hour with ab322307 at 1/100 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com