Alexa Fluor® 647 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (AB202639)

ab202639 staining Wilms Tumor Protein in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202639 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (AB202639)

Immunohistochemical analysis of paraffin-embedded rat testis labeling Wilms Tumor Protein with ab202639 at 1/50 dilution.

Positive staining on rat testis.
The section was incubated with ab202639 for 60 mins at room temperature (shown in red).
Nuclear DNA was labeled with DAPI (shown in blue).
The section was then mounted using Fluoromount^®.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (AB202639)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle labeling Wilms Tumor Protein with ab202639 at 1/50 dilution.

Negative control : no staining on mouse skeletal muscle.
The section was incubated with ab202639 for 60 mins at room temperature (shown in red).
Nuclear DNA was labeled with DAPI (shown in blue).
The section was then mounted using Fluoromount^®.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (AB202639)

Immunohistochemical analysis of paraffin-embedded mouse testis labeling Wilms Tumor Protein with ab202639 at 1/50 dilution.

Positive staining on mouse testis.
The section was incubated with ab202639 for 60 mins at room temperature (shown in red).
Nuclear DNA was labeled with DAPI (shown in blue).
The section was then mounted using Fluoromount^®.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.