AB184093

Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control

Name: Anti-alpha Tubulin Alexa Fluor® 680 antibody [DM1A] (ab184093) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB184093

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(1 Publication)

Mouse Monoclonal Alpha-tubulin 4 antibody - conjugated to Alexa Fluor® 680. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

1 Images

Unknown

Western blot - Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB184093)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184093 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184093) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Predicted band size: 50 kDa

Observed band size: 52 kDa

false

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker
HRP

HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control
519 FITC

FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker
805 Alexa Fluor® 790

Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker
Carrier free

Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free
Unconjugated

Anti-alpha Tubulin antibody [DM1A] - Loading Control

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DM1A

Isotype

IgG1

Light chain type

kappa

Conjugation

Alexa Fluor® 680

Excitation/Emission

Ex: 679nm, Em: 702nm

Carrier free

Reacts with

Mouse, Human

Applications

applications

Epitope

aa 426-450

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.

Biological function summary

Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.

Pathways

Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.

Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA4A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature chemical biology 21:736-745 PubMed40133642

2025

Pharmacological targeting of BMAL1 modulates circadian and immune pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Pu,Laura C Bailey,Ludwig G Bauer,Maria Voronkov,Matthew Baxter,Kilian V M Huber,Sepideh Khorasanizadeh,David Ray,Fraydoon Rastinejad

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control

Western blot - Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB184093)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Epitope

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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