AB184092

Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control

Name: Anti-beta Actin Alexa Fluor® 680 antibody [mAbcam 8226] (ab184092) | Abcam
Brand: Abcam Limited
SKU: AB184092
Price: 460 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal beta Actin antibody - conjugated to Alexa Fluor® 680. Suitable for WB and reacts with Mouse, Human samples. Cited in 7 publications.

View Alternative Names

Beta-actin, ACTB

1 Images

Unknown

Western blot - Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB184092)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184092 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184092) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Predicted band size: 41 kDa

Observed band size: 42 kDa

false

Carrier free

Anti-beta Actin antibody [mAbcam 8226] - BSA and Azide free
Unconjugated

Anti-beta Actin antibody [mAbcam 8226] - Loading Control
805 Alexa Fluor® 790

Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control
HRP

HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam 8226

Isotype

IgG1

Light chain type

kappa

Conjugation

Alexa Fluor® 680

Excitation/Emission

Ex: 679nm, Em: 702nm

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (PubMed : 39321809, PubMed : 38609661). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

See full target information ACTB

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:6628-6650 PubMed40585999

2025

Gut microbiota depletion accelerates hematoma resolution and neurological recovery after intracerebral hemorrhage via p-coumaric acid-promoted Treg differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yonghe Zheng,Yin Li,Xuchao He,Yuhan Zhu,Shiyu Xu,Yirong Feng,Yirui Kuang,Huaijun Chen,Linfeng Fan,Huaping Huang,Libin Hu,Xian Yu,Jianan Wu,Lingji Jin,Junwen Hu,Xiongjie Fu,Hanhai Zeng,Shenglong Cao,Lin Wang

Nature chemical biology 21:736-745 PubMed40133642

2025

Pharmacological targeting of BMAL1 modulates circadian and immune pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Pu,Laura C Bailey,Ludwig G Bauer,Maria Voronkov,Matthew Baxter,Kilian V M Huber,Sepideh Khorasanizadeh,David Ray,Fraydoon Rastinejad

Cells 13: PubMed39451222

2024

Decreased Circulating Gonadotropin-Releasing Hormone Associated with Keratoconus.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Escandon,Alexander J Choi,Steve Mabry,Sarah E Nicholas,Rebecca L Cunningham,Liam Redden,David A Murphy,Kamran M Riaz,Tina B McKay,Dimitrios Karamichos

Molecular biology of the cell 35:ar140 PubMed39356796

2024

G3BP isoforms differentially affect stress granule assembly and gene expression during cellular stress.

Applications

Unspecified application

Species

Unspecified reactive species

José M Liboy-Lugo,Carla A Espinoza,Jessica Sheu-Gruttadauria,Jesslyn E Park,Albert Xu,Ziad Jowhar,Angela L Gao,José A Carmona-Negrón,Torsten Wittmann,Natalia Jura,Stephen N Floor

International journal of molecular sciences 25: PubMed38928268

2024

Amelioration of Fibrosis via S1P Inhibition Is Regulated by Inactivation of TGF-β and SPL Pathways in the Human Cornea.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Nicholas,Sandip K Basu,Nawajes Mandal,Dimitrios Karamichos

The American journal of pathology 193:1762-1775 PubMed36822267

2023

Selective Modulation of the Keratoconic Stromal Microenvironment by FSH and LH.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Escandon,Sarah E Nicholas,Brenda Vasini,Rebecca L Cunningham,David A Murphy,Kamran M Riaz,Dimitrios Karamichos

International journal of molecular sciences 23: PubMed35457149

2022

Unravelling Novel Roles of Salivary Exosomes in the Regulation of Human Corneal Stromal Cell Migration and Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Escandon,Angela Liu,Sarah E Nicholas,Asher Khan,Kamran M Riaz,Dimitrios Karamichos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control

Western blot - Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB184092)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise