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Mouse Monoclonal CD33 antibody - conjugated to Alexa Fluor® 700. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD33 protein.

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Images

Flow Cytometry - Alexa Fluor® 700 Anti-CD33 antibody [WM53] (AB269306), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

Alexa Fluor® 700

Excitation/Emission

Ex: 702nm, Em: 723nm

Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing CD33 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P20138

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested
Primates
Predicted

Tested
Tested

Species

Human

Dilution info

4 µL for 106 Cells

Notes

Or 100 μL of whole blood.

Predicted
Predicted

Species

Primates

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:11320212, PubMed:15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:10887109, PubMed:28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10206955, PubMed:10556798, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).

Alternative names

Recommended products

Mouse Monoclonal CD33 antibody - conjugated to Alexa Fluor® 700. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD33 protein.

Key facts

Isotype

IgG1

Conjugation

Alexa Fluor® 700

Excitation/Emission

Ex: 702nm, Em: 723nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing CD33 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P20138
Clone number

WM53

Purification technique

Size-exclusion chromatography

Concentration
Loading...
Purification notes

The purified antibody is conjugated with Alexa Fluor® 700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

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1 product image

  • Flow Cytometry - Alexa Fluor® 700 Anti-CD33 antibody [WM53] (ab269306), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 700 Anti-CD33 antibody [WM53] (ab269306)

    Flow cytometry analysis (surface staining) of human peripheral blood labeling CD33 with ab269306.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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