Mouse Monoclonal CD33 antibody - conjugated to Alexa Fluor® 700. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD33 protein.
IgG1
Mouse
Alexa Fluor® 700
Ex: 702nm, Em: 723nm
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS
Liquid
Monoclonal
Flow Cyt | |
---|---|
Human | Tested |
Primates | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 4 µL for 106 Cells | Notes Or 100 μL of whole blood. |
Species | Dilution info | Notes |
---|---|---|
Species Primates | Dilution info - | Notes - |
Select an associated product type
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:11320212, PubMed:15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:10887109, PubMed:28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10206955, PubMed:10556798, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).
SIGLEC3, CD33, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3
Mouse Monoclonal CD33 antibody - conjugated to Alexa Fluor® 700. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD33 protein.
IgG1
Mouse
Alexa Fluor® 700
Ex: 702nm, Em: 723nm
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS
Liquid
Monoclonal
WM53
Size-exclusion chromatography
The purified antibody is conjugated with Alexa Fluor® 700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Blue Ice
+4°C
+4°C
Store in the dark
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Flow cytometry analysis (surface staining) of human peripheral blood labeling CD33 with ab269306.
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