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AB270669

Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]]

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Mouse Monoclonal CD34 antibody - conjugated to Alexa Fluor® 700. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD34 protein.

View Alternative Names

CD34, Hematopoietic progenitor cell antigen CD34

3 Images
Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (AB270669)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (AB270669)

Flow cytometry analysis of human peripheral whole blood labeling CD34 using ab270669. Surface staining.

Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (AB270669)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (AB270669)

Flow cytometry analysis of human peripheral whole blood labeling CD34 positive stem cells using ab270669 (Red).

Control : Lymphocytes (Black).

Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (AB270669)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (AB270669)

Flow cytometry analysis of human peripheral whole blood labeling CD34 positive stem cells using ab270669 (Red). Surface staining.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4H11[APG]

Isotype

IgG1

Conjugation

Alexa Fluor® 700

Excitation/Emission

Ex: 702nm, Em: 723nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD34 protein. The exact immunogen used to generate this antibody is proprietary information.

P28906

Specificity

This antibody reacts with extracellular class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of class II antibody QBEnd10 and class III antibodies BIRMA K3 and 8G12 on KG1a cell line.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in suspension.</p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
See full target information CD34

Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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