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AB275952

Alexa Fluor® 700 Anti-IL3RA/CD123 antibody [6H6]

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Mouse Monoclonal IL3RA/CD123 antibody - conjugated to Alexa Fluor® 700. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing IL3RA protein.

View Alternative Names

CD123, IL3R, IL3RA, Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA

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Flow Cytometry - Alexa Fluor® 700 Anti-IL3RA/CD123 antibody [6H6] (AB275952)
  • Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 700 Anti-IL3RA/CD123 antibody [6H6] (AB275952)

Separation of human CD123 positive cells (red-filled) from CD123 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab275952 (4 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - Alexa Fluor® 700 Anti-IL3RA/CD123 antibody [6H6] (AB275952)
  • Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 700 Anti-IL3RA/CD123 antibody [6H6] (AB275952)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab275952 (4 μl reagent / 100 μl of peripheral whole blood).

  • Unconjugated

    Anti-IL3RA/CD123 antibody [6H6]

  • 778 PE/Cy7®

    PE/Cy7® Anti-IL3RA/CD123 antibody [6H6]

  • 578 PE

    PE Anti-IL3RA/CD123 antibody [6H6]

  • Biotin

    Biotin Anti-IL3RA/CD123 antibody [6H6]

  • 660 APC

    APC Anti-IL3RA/CD123 antibody [6H6]

  • 519 FITC

    FITC Anti-IL3RA/CD123 antibody [6H6]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-IL3RA/CD123 antibody [6H6]

  • 667 PE/Cy5®

    PE/Cy5® Anti-IL3RA/CD123 antibody [6H6]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6H6

Isotype

IgG1

Conjugation

Alexa Fluor® 700

Excitation/Emission

Ex: 702nm, Em: 723nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing IL3RA protein. The exact immunogen used to generate this antibody is proprietary information.

P26951

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>4 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension.</p>" } } }
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You may be interested in:

AB300317

Human IL3RA/CD123 ELISA Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: 99.9% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL3RA also known as CD123 or interleukin-3 receptor alpha chain is a transmembrane protein with a molecular weight of approximately 70-80 kDa. This protein frequently expresses on the surface of hematopoietic cells particularly those of myeloid lineage including mast cells and basophils. Researchers use CD123 as a marker for identifying certain cell types in flow cytometry. Antibodies such as anti-CD123 are available for various applications including identifying these cells in research and clinical settings.
Biological function summary

IL3RA forms part of a heterodimeric receptor complex binding with the beta-common (βc) chain to create the high-affinity IL-3 receptor. This complex plays a role in mediating the effects of interleukin-3 a cytokine that promotes the growth and differentiation of pluripotent hematopoietic stem cells. Through this mechanism the receptor influences immune response modulation particularly in allergic reactions and inflammation.

Pathways

IL3RA interacts with the JAK/STAT pathway which is essential for signal transduction and transcription activation in response to IL-3 binding. This pathway involves proteins like Janus kinase 2 (JAK2) and Signal Transducer and Activator of Transcription 5 (STAT5) which transmit signals from IL3RA engagement to the cell nucleus promoting gene expression associated with cell proliferation and survival.

IL3RA expression relates closely to certain hematological conditions such as acute myeloid leukemia (AML) and systemic mastocytosis. In AML increased expression of CD123 marks leukemic cells making it a target for therapeutic intervention. The interaction of IL3RA with FLT3 another tyrosine kinase is of particular interest in these disorders as both proteins contribute to the proliferation of malignant cells through related signaling pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface receptor for IL3 expressed on hematopoietic progenitor cells, monocytes and B-lymphocytes that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed : 10527461). Ligand stimulation rapidly induces hetrodimerization with IL3RB, phosphorylation and enzyme activity of effector proteins such as JAK2 and PI3K that play a role in signaling cell proliferation and differentiation. Activation of JAK2 leads to STAT5-mediated transcriptional program (By similarity).
See full target information IL3RA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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