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AB321359

Alexa Fluor® 750 Anti-active YAP1 antibody [EPR19812]

1

(1 Review)

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(0 Publication)

Rabbit Recombinant Monoclonal YAP1 antibody - conjugated to Alexa Fluor® 750.

View Alternative Names

YAP65, YAP1, Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19812

Isotype

IgG

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab205270 (parent) is specific to the active (non-phosphorylated) form of YAP1.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Active YAP1 also known as Yes-associated protein 1 is a transcription co-activator with a mass of approximately 65 kDa. It plays a mechanical role in the regulation of gene expression by interacting with transcription factors and enhancing their ability to drive transcription. YAP1 is expressed in various tissues including the liver intestine and neural tissues. Also found in the cytoplasm and nucleus its expression is tightly regulated because of its involvement in maintaining cell proliferation and survival.
Biological function summary

Active YAP1 functions as an important regulator in the Hippo signaling pathway. It acts as an effector that transmits signals from upstream kinases to control organ size by modulating cell growth and apoptosis. YAP1 associates with the TEAD transcription factor family to regulate the expression of genes involved in cell proliferation and differentiation. The complex formed between YAP1 and TEAD is essential for its biological activity influencing processes like stem cell renewal and tissue regeneration.

Pathways

Active YAP1 has a central role in the Hippo signaling pathway important for controlling cellular behaviors such as growth and survival. In this pathway YAP1 interacts with other proteins like LATS1/2 kinases to control its activity through phosphorylation and subcellular localization. Akt/mTOR pathway also connects with YAP1 integrating signals that affect cell growth and metabolism. These interactions highlight YAP1’s position as a pivotal node in maintaining cellular homeostasis.

Active YAP1 is implicated in several conditions including cancer and liver disorders. Overexpression or dysregulation of YAP1 leads to oncogenic transformation closely associating it with cancers such as hepatocellular carcinoma and breast cancer. In liver disorders YAP1's activity affects fibrosis and regeneration by interacting with proteins like β-catenin modulating pathways linked to tissue repair and scarring. Understanding YAP1’s role offers potential therapeutic targets for managing these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the YAP1 gene functions as a transcriptional regulator, acting both as a coactivator and corepressor. It is a critical downstream regulatory target in the Hippo signaling pathway, which is crucial for organ size control and tumor suppression, as it restricts proliferation and promotes apoptosis. The pathway involves a kinase cascade where STK3/MST2 and STK4/MST1 activate LATS1/2, which then inactivates the YAP1 oncoprotein. YAP1 plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation through ARHGAP18. It controls cell proliferation in response to cell contact, with its phosphorylation by LATS1/2 preventing nuclear translocation necessary for regulating cellular genes linked to proliferation, death, and migration. TEAD transcription factors are needed for YAP1 to stimulate gene expression, cell growth, anchorage-independent growth, and EMT induction. Additionally, YAP1 suppresses ciliogenesis by repressing TEAD4 target genes AURKA and PLK1. Isoforms 2 and 3 of YAP1 activate the C-terminal fragment of ERBB4 (isoform 3). This supplementary information is collated from multiple sources and compiled automatically.
See full target information YAP1
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Product promise

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