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AB321167

Alexa Fluor® 750 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

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Rabbit Recombinant Monoclonal Aspartate Aminotransferase antibody - conjugated to Alexa Fluor® 750.

View Alternative Names

cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • HRP

    HRP Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 578 PE

    PE Anti-Aspartate Aminotransferase +FABP-1 antibody [EPR12145]

  • 660 APC

    APC Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • Unconjugated

    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • Carrier free

    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12145

Isotype

IgG

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation on the parent is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate Aminotransferase (AST) also known as glutamate oxaloacetate transaminase is an enzyme that plays an important role in amino acid metabolism. AST transfers an amino group typically from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. This enzyme approximately weighs 45 kDa. AST is widely expressed in tissues with especially high levels found in the liver heart skeletal muscle and kidneys.
Biological function summary

Aspartate aminotransferase participates in the malate-aspartate shuttle which facilitates the transfer of reducing equivalents across the mitochondrial membrane. This shuttle system is important in maintaining the balance of NADH and NAD+ between the cytosol and mitochondria. AST forms part of a protein complex that includes α-ketoglutarate and oxaloacetate which are central to its transaminase activity in cellular metabolism.

Pathways

Aspartate aminotransferase is significantly involved in the tricarboxylic acid (TCA) cycle and the urea cycle. Within the TCA cycle AST ensures the linkage between carbohydrate and amino acid metabolism by converting aspartate and α-ketoglutarate into oxaloacetate and glutamate. In the urea cycle the enzyme connects to argininosuccinate synthetase and argininosuccinate lyase facilitating the removal of excess nitrogen from the body.

Elevated levels of aspartate aminotransferase are often indicative of liver damage including conditions like hepatitis and cirrhosis. Additionally the enzyme associates with myocardial infarction due to its high activity in cardiac tissue. Liver-related disorders may also involve fatty acid-binding protein 1 (FABP-1) which closely interacts with AST and serves as a marker for liver function and damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed : 21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed : 27827456).
See full target information Aspartate aminotransferase, cytoplasmic

Additional targets

GOT2
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Product promise

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