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AB320894

Alexa Fluor® 750 Anti-ATG9A antibody [EPR2450(2)]

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Rabbit Recombinant Monoclonal ATG9A antibody - conjugated to Alexa Fluor® 750.

View Alternative Names

APG9L1, ATG9A, Autophagy-related protein 9A, APG9-like 1, mATG9

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-ATG9A antibody [EPR2450(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-ATG9A antibody [EPR2450(2)]

  • 660 APC

    APC Anti-ATG9A antibody [EPR2450(2)]

  • 578 PE

    PE Anti-ATG9A antibody [EPR2450(2)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-ATG9A antibody [EPR2450(2)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-ATG9A antibody [EPR2450(2)]

  • Unconjugated

    Anti-ATG9A antibody [EPR2450(2)]

  • Carrier free

    Anti-ATG9A antibody [EPR2450(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2450(2)

Isotype

IgG

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation on the parent is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG9A also known as APG9-like 1 or ATG9 autophagy related 9A is an important component in the autophagy machinery. This protein has a molecular weight of approximately 93 kDa. It is ubiquitously expressed in various tissues with higher expression seen in the heart and skeletal muscle. Mechanically ATG9A is essential for the trafficking of membranes necessary for autophagosome formation. It actively participates in membrane lipids' delivery from donor organelles which are critical for constructing autophagosomes.
Biological function summary

ATG9A facilitates the recycling of cellular materials through a process called autophagy. This protein acts with other autophagy-related proteins to form a complex essential for autophagosome elongation and closure. The ATG9A protein's dynamic movements between the Golgi apparatus and endosomes ensure proper membrane supply for autophagy. It coordinates with the ULK1 complex and various phospholipid-modifying enzymes which are necessary to regulate autophagic flux.

Pathways

ATG9A plays an integral role in the mTOR signaling and MAPK pathways. Both pathways are essential for cellular responses to stress and nutrient availability. In these pathways ATG9A works closely with proteins like beclin 1 and ATG5. Inhibiting mTOR actively induces autophagy where ATG9A contributes to membrane recruitment and elongation of the autophagosomes facilitating the clearance of damaged organelles and proteins.

ATG9A shows a significant link to neurodegenerative diseases such as Parkinson's and Alzheimer's disease. The dysfunction of autophagic pathways due to altered ATG9A function leads to the accumulation of damaged proteins and organelles. This event contributes to the pathogenesis of such neurodegenerative diseases. Moreover ATG9A closely associates with proteins like LAMP2 whose mutations similarly lead to defective autophagic processes resulting in the accumulation of unprocessed cellular debris in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion (PubMed : 22456507, PubMed : 27510922, PubMed : 29437695, PubMed : 32513819, PubMed : 32610138, PubMed : 33106659, PubMed : 33468622, PubMed : 33850023). Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome (PubMed : 16940348, PubMed : 22456507, PubMed : 33106659). Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion (PubMed : 33106659). Also required to supply phosphatidylinositol 4-phosphate to the autophagosome initiation site by recruiting the phosphatidylinositol 4-kinase beta (PI4KB) in a process dependent on ARFIP2, but not ARFIP1 (PubMed : 30917996). In addition to autophagy, also plays a role in necrotic cell death (By similarity).
See full target information ATG9A
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Product promise

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