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AB321242

Alexa Fluor® 750 Anti-EAAT1 antibody [EPR12686]

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Rabbit Recombinant Monoclonal EAAT1 antibody - conjugated to Alexa Fluor® 750.

View Alternative Names

EAAT1, GLAST, GLAST1, SLC1A3, Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1, Solute carrier family 1 member 3, GLAST-1

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12686

Isotype

IgG

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Unsuitable for human ICC/IF.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EAAT1 also known as Excitatory Amino Acid Transporter 1 or anti-GLAST plays a critical role in mediating the uptake of glutamate from the synaptic cleft into glial cells. This transporter helps maintain the delicate balance of excitatory neurotransmission in the central nervous system. EAAT1 has a molecular mass of approximately 57 kDa. You will find EAAT1 expressed predominantly in astrocytes within the brain and retina where it facilitates the regulation of extracellular concentrations of glutamate preventing excitotoxicity.
Biological function summary

EAAT1 is instrumental in maintaining low levels of extracellular glutamate acting as a protective mechanism against neurotoxicity. This transporter does not operate as part of a larger protein complex instead functioning independently to efficiently clear synaptic glutamate. By ensuring rapid uptake of glutamate EAAT1 helps maintain normal synaptic transmission and neuronal communication. The action of EAAT1 is important for preventing excessive activation of glutamate receptors which can lead to cellular damage.

Pathways

EAAT1 is heavily involved in the glutamatergic neurotransmission pathway which is essential for various brain functions including learning and memory. EAAT1 acts in coordination with other proteins like EAAT2 to manage glutamate levels within the synaptic cleft. Another pathway where EAAT1 plays a role is the glutamate-glutamine cycle where it works alongside proteins such as glutamine synthetase to recycle glutamate and sustain neurotransmitter balance. This interplay supports neuronal health and protects against glutamate excitotoxicity.

EAAT1 has been implicated in neurological conditions such as epilepsy and Alzheimer's disease. The dysfunction or altered expression of EAAT1 can lead to insufficient glutamate clearance contributing to the pathophysiology of these disorders. In epilepsy the decreased function of EAAT1 may result in abnormally high levels of synaptic glutamate enhancing the risk of seizures. Similarly in Alzheimer's altered EAAT1 activity can exacerbate neurodegenerative processes. EAAT1's role is tightly linked with proteins like NMDA receptors and EAAT2 which further influence disease progression by affecting glutamate dynamics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed : 20477940, PubMed : 26690923, PubMed : 28032905, PubMed : 28424515, PubMed : 7521911, PubMed : 8123008). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed : 20477940). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed : 20477940). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity).
See full target information SLC1A3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com