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AB321339

Alexa Fluor® 750 Anti-GOLPH4/GPP130 antibody [EPR13439]

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Rabbit Recombinant Monoclonal GOLPH4/GPP130 antibody - conjugated to Alexa Fluor® 750. Suitable for Target Binding Affinity, Antibody Labelling, IHC-P and reacts with Human samples.

View Alternative Names

GIMPC, GOLPH4, GPP130, GOLIM4, Golgi integral membrane protein 4, Golgi phosphoprotein 4, Golgi-localized phosphoprotein of 130 kDa, GIMPc, Golgi phosphoprotein of 130 kDa

  • 660 APC

    APC Anti-GOLPH4/GPP130 antibody [EPR13439]

  • 578 PE

    PE Anti-GOLPH4/GPP130 antibody [EPR13439]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-GOLPH4/GPP130 antibody [EPR13439]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-GOLPH4/GPP130 antibody [EPR13439]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-GOLPH4/GPP130 antibody [EPR13439]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-GOLPH4/GPP130 antibody [EPR13439]

  • Unconjugated

    Anti-GOLPH4/GPP130 antibody [EPR13439]

  • Carrier free

    Anti-GOLPH4/GPP130 antibody [EPR13439] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13439

Isotype

IgG

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Carrier free

No

Reacts with

Human

Applications

Target Binding Affinity, Antibody Labelling, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Proteins & Peptides

AB162146

Recombinant Human GOLPH4/GPP130 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human GOLPH4/GPP130 protein (GST tag N-Terminus) (AB162146)

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Cell Lines & Lysates

AB266123

Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line

Advanced Validation

Western blot - Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line (AB266123)

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Cell Lines & Lysates

AB257455

Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell lysate

Western blot - Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell lysate (AB257455)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GOLPH4 also known as GPP130 and Golgi integral membrane protein 4 is a protein with a mass of approximately 130 kDa. It plays an important role in the organization and maintenance of the Golgi apparatus structure. GOLPH4 is predominantly found in the cis-Golgi network and it is involved in the cellular sorting and trafficking of proteins and lipids. The protein functions as a tethering factor and it may participate in the transport of cargo from the Golgi to other cellular locations.
Biological function summary

The role of GOLPH4 centers around its involvement in the protein trafficking process within cells. It acts as part of the complex tasked with maintaining Golgi organization which is important for facilitating accurate cargo sorting and delivery. GOLPH4's location and structure enable it to interact with motor proteins and other Golgi matrix proteins aiding in vesicle tethering and fusion processes.

Pathways

GOLPH4 is involved in essential cellular pathways such as the secretion and endocytic trafficking pathways. It interacts with other Golgi-resident proteins like GM130 and giantin which are essential for the movement of materials through the Golgi and to their final destinations. These pathways ensure that proteins and lipids reach their intended areas within the cell or are secreted to the extracellular space which is important for maintaining cellular function and homeostasis.

Disturbances in GOLPH4 function have been associated with conditions such as cancer and neurodegenerative diseases. Aberrant expression or mutations in this protein can lead to misregulation of Golgi functions affecting overall cellular trafficking systems. GOLPH4's interaction with proteins involved in these conditions such as p53 in cancer or tau in neurodegenerative diseases highlights its potential as a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.
See full target information GOLIM4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com