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AB321401

Alexa Fluor® 750 Anti-HDAC1 + HDAC2 antibody [EPR20327]

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Rabbit Recombinant Monoclonal HDAC2 antibody - conjugated to Alexa Fluor® 750.

View Alternative Names

Histone deacetylase 2, HD2, Protein deacylase HDAC2, HDAC2

  • Unconjugated

    Anti-HDAC1 + HDAC2 antibody [EPR20327]

  • Carrier free

    Anti-HDAC1+HDAC2 antibody [EPR20327] - BSA and Azide free

  • 578 PE

    PE Anti-HDAC1 + HDAC2 antibody [EPR20327]

  • 660 APC

    APC Anti-HDAC1 + HDAC2 antibody [EPR20327]

  • HRP

    HRP Anti-HDAC1 + HDAC2 antibody [EPR20327]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-HDAC1 + HDAC2 antibody [EPR20327]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-HDAC1 + HDAC2 antibody [EPR20327]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-HDAC1 + HDAC2 antibody [EPR20327]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-HDAC1 + HDAC2 antibody [EPR20327]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-HDAC1 + HDAC2 antibody [EPR20327]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20327

Isotype

IgG

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric)

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC1 and HDAC2 also known as histone deacetylase 1 and 2 are enzymes that remove acetyl groups from histone proteins leading to chromatin condensation and transcriptional repression. HDAC1 has a molecular weight of approximately 62 kDa and is expressed in various cell types including those in the central nervous system heart and immune cells. HDAC2 shares similar expression patterns with a molecular weight around 55 kDa. Both proteins play a role in regulating gene expression by altering chromatin structure.
Biological function summary

Both HDAC1 and HDAC2 are components of large multiprotein complexes such as the Sin3 NuRD and CoREST complexes which are involved in transcriptional regulation. These complexes facilitate the deacetylation of histones transcription factors and other cellular proteins influencing cell cycle progression differentiation and development. HDAC1 and HDAC2 modulate cellular activities impacting cell proliferation and survival.

Pathways

HDAC1 and HDAC2 participate in key cellular processes particularly the cell cycle and apoptosis pathways. These enzymes closely interact with proteins like pRb and E2F influencing the transition between different cell cycle phases. HDAC1 and HDAC2 also connect with tumor suppressor protein p53 affecting apoptotic pathways. By modulating these pathways they play an essential role in maintaining cellular homeostasis.

HDAC1 and HDAC2 are pertinent to certain cancers and neurodegenerative diseases. Their aberrant activity links to cancer progression influencing genes involved in cell cycle regulation. Overexpression or dysregulation may contribute to the development of cancer types such as leukemia. In neurodegenerative diseases particularly Huntington’s disease abnormal HDAC1 and HDAC2 activity impacts pathways involving neuroplasticity and neuron survival. Shared interactions with proteins like p53 highlight their significance in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed : 28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (By similarity). Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR (PubMed : 12724404). Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (By similarity). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 28977666). Component of the SIN3B complex that represses transcription and counteracts the histone acetyltransferase activity of EP300 through the recognition H3K27ac marks by PHF12 and the activity of the histone deacetylase HDAC2 (PubMed : 37137925). Also deacetylates non-histone targets : deacetylates TSHZ3, thereby regulating its transcriptional repressor activity (PubMed : 19343227). May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation (By similarity). Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A (PubMed : 21965678). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups : catalyzes removal of (2E)-butenoyl (crotonyl), lactoyl (lactyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation, delactylation and de-2-hydroxyisobutyrylation, respectively (PubMed : 28497810, PubMed : 29192674, PubMed : 35044827).
See full target information HDAC2

Additional targets

HDAC1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com