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AB320976

Alexa Fluor® 750 Anti-IRF2 antibody [EPR4644(2)]

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Rabbit Recombinant Monoclonal IRF2 antibody - conjugated to Alexa Fluor® 750. Suitable for Target Binding Affinity, Antibody Labelling, ICC/IF, IHC-P and reacts with Human, Mouse samples.

View Alternative Names

Interferon regulatory factor 2, IRF-2, IRF2

  • 578 PE

    PE Anti-IRF2 antibody [EPR4644(2)]

  • 660 APC

    APC Anti-IRF2 antibody [EPR4644(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-IRF2 antibody [EPR4644(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-IRF2 antibody [EPR4644(2)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-IRF2 antibody [EPR4644(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-IRF2 antibody [EPR4644(2)]

  • Unconjugated

    Anti-IRF2 antibody [EPR4644(2)]

  • Carrier free

    Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4644(2)

Isotype

IgG

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Carrier free

No

Reacts with

Human, Mouse

Applications

Target Binding Affinity, ICC/IF, IHC-P, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Cell Lines & Lysates

AB267016

Human IRF2 knockout A549 cell line

Sanger Sequencing - Human IRF2 knockout A549 cell line (AB267016)

  • 0
  • 0
Proteins & Peptides

AB59954

Recombinant Human IRF2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human IRF2 protein (His tag N-Terminus) (AB59954)

  • 0
  • 0
Cell Lines & Lysates

AB258007

Human IRF2 knockout A549 cell lysate

Sanger Sequencing - Human IRF2 knockout A549 cell lysate (AB258007)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Interferon Regulatory Factor 2 (IRF2) also known as IRF-2 plays an important role in cellular processes like modulation of immune responses and regulation of gene expression. It has a molecular mass of approximately 39 kDa. IRF2 is expressed across various tissues including the immune system cells fibroblasts and epithelial cells. It can function as both a transcriptional activator and repressor depending on the context and specific gene targets.
Biological function summary

IRF2 regulates immune responses by controlling the expression of interferon (IFN) responsive genes. It acts independently and as part of transcriptional complexes binding to specific DNA sequences within target genes. Its action prevents excessive activation of immune responses maintaining a balanced immune system. IRF2 also influences cell cycle regulation contributing to its role in cell growth and differentiation.

Pathways

And several cellular communication networks IRF2 is a central figure in the interferon signaling pathway interacting with other members of the IRF family like IRF1 and IRF3. These interactions help propagate signals necessary for effective immune responses. In conjunction with the JAK-STAT pathway IRF2 modulates expressions that control antiviral responses and cellular proliferation showing its critical placement in immune response regulation.

IRF2's dysregulation has associations with cancer and autoimmune diseases. Overexpression of IRF2 is linked to certain cancers providing cells with growth advantages and resistance to apoptosis commonly in concert with proteins like STAT1. In autoimmune diseases IRF2 impacts inflammatory responses with aberrant regulation contributing to conditions like systemic lupus erythematosus. Its involvement highlights the complex nature of immune regulation and potential for targeted therapy interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-binding transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible genes and regulates their expression (PubMed : 39013473, PubMed : 7687740). Mainly acts as a transcription repressor, repressing expression (PubMed : 39013473). Also acts as an activator for several genes including H4 and IL7 (PubMed : 15226432, PubMed : 9540062). Constitutively binds to the ISRE promoter to activate IL7 (PubMed : 15226432). Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth (PubMed : 9540062). Antagonizes IRF1 transcriptional activation (By similarity).. Isoform 2. Unable to bind to IRF2BP1 and IRF2BP2 corepressors and cannot mediate repression.
See full target information IRF2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com