AB320915

Alexa Fluor® 750 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal Lamin B1 antibody - conjugated to Alexa Fluor® 750. Suitable for Target Binding Affinity, Antibody Labelling, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

LMN2, LMNB, LMNB1, Lamin-B1, LMN1, LMNA, Prelamin-A/C, LMN1, LMNA, Prelamin-A/C

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
578 PE

PE Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
660 APC

APC Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
HRP

HRP Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
Unconjugated

Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
Carrier free

Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4068

Isotype

IgG

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Carrier free

Reacts with

Human, Mouse, Rat

Applications

Antibody Labelling, ICC/IF, IHC-P, Target Binding Affinity, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody recognizes full length Lamin A/B1/C and the cleaved small unit.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lamin A Lamin B1 and Lamin C are nuclear envelope proteins critical for nuclear structure and stability. Lamin A and Lamin C both originating from the LMNA gene have molecular masses of about 74 kDa and 65 kDa respectively due to alternative splicing. Lamin B1 encoded by the LMNB1 gene has a molecular mass of around 67 kDa. These lamins are found in the nuclear lamina a dense fibrillar network lining the inner surface of the nuclear envelope. They anchor chromatin to the nuclear envelope and play roles in DNA replication repair and gene expression.

Biological function summary

Lamins play essential roles in maintaining cell integrity and regulating gene expression. Lamin A and Lamin C part of the type A lamins and Lamin B1 part of the type B lamins contribute to nuclear organization and mechanical support. These proteins form complexes within the lamina network and with other nuclear proteins participating in chromatin organization and gene transcription. Defects in lamins negatively impact cellular functions and can lead to altered gene expression patterns.

Pathways

Lamins interact with multiple signaling pathways that govern cellular homeostasis and stress response. They play roles in the mechanotransduction pathway influencing how cells perceive mechanical stress and respond to it. Interaction with proteins like emerin and nuclear actin further connects lamins to pathways involved in gene regulation and structural support of the nucleus.

Lamin dysfunction has associations with several human diseases including laminopathies such as Hutchinson-Gilford progeria syndrome and muscular dystrophy. Mutations in the LMNA gene affecting Lamin A and Lamin C often lead to disrupted nuclear architecture and impaired cellular function. Lamin B1 when overexpressed or mutated has links to neurological disorders like autosomal dominant leukodystrophy. These associations highlight the importance of lamins in disease mechanisms and provide insights into potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed : 28716252, PubMed : 32910914). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed : 28716252, PubMed : 32910914). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed : 28716252, PubMed : 32910914).

See full target information LMNB1

Additional targets

LMNA,LMNA

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 750 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise