Rabbit Recombinant Monoclonal Lamin B1 antibody - conjugated to Alexa Fluor® 750.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:28716252, PubMed:32910914). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:28716252, PubMed:32910914). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:28716252, PubMed:32910914).
LMNA, LMNA
LMN2, LMNB, LMNB1, Lamin-B1
Rabbit Recombinant Monoclonal Lamin B1 antibody - conjugated to Alexa Fluor® 750.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
The antibody recognizes full length Lamin A/B1/C and the cleaved small unit.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Lamin A Lamin B1 and Lamin C are nuclear envelope proteins critical for nuclear structure and stability. Lamin A and Lamin C both originating from the LMNA gene have molecular masses of about 74 kDa and 65 kDa respectively due to alternative splicing. Lamin B1 encoded by the LMNB1 gene has a molecular mass of around 67 kDa. These lamins are found in the nuclear lamina a dense fibrillar network lining the inner surface of the nuclear envelope. They anchor chromatin to the nuclear envelope and play roles in DNA replication repair and gene expression.
Lamins play essential roles in maintaining cell integrity and regulating gene expression. Lamin A and Lamin C part of the type A lamins and Lamin B1 part of the type B lamins contribute to nuclear organization and mechanical support. These proteins form complexes within the lamina network and with other nuclear proteins participating in chromatin organization and gene transcription. Defects in lamins negatively impact cellular functions and can lead to altered gene expression patterns.
Lamins interact with multiple signaling pathways that govern cellular homeostasis and stress response. They play roles in the mechanotransduction pathway influencing how cells perceive mechanical stress and respond to it. Interaction with proteins like emerin and nuclear actin further connects lamins to pathways involved in gene regulation and structural support of the nucleus.
Lamin dysfunction has associations with several human diseases including laminopathies such as Hutchinson-Gilford progeria syndrome and muscular dystrophy. Mutations in the LMNA gene affecting Lamin A and Lamin C often lead to disrupted nuclear architecture and impaired cellular function. Lamin B1 when overexpressed or mutated has links to neurological disorders like autosomal dominant leukodystrophy. These associations highlight the importance of lamins in disease mechanisms and provide insights into potential therapeutic targets.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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