Rabbit Recombinant Monoclonal N WASP antibody - conjugated to Alexa Fluor® 750.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex (PubMed:16767080, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828, PubMed:9422512). Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization (PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828, PubMed:9422512). Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia (PubMed:9422512). In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization (PubMed:16767080). Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (By similarity). Plays a role in dendrite spine morphogenesis (By similarity). Decreasing levels of DNMBP (using antisense RNA) alters apical junction morphology in cultured enterocytes, junctions curve instead of being nearly linear (PubMed:19767742).
Actin nucleation-promoting factor WASL, Neural Wiskott-Aldrich syndrome protein, N-WASP, WASL
Rabbit Recombinant Monoclonal N WASP antibody - conjugated to Alexa Fluor® 750.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The protein N-WASP also known as neural Wiskott-Aldrich syndrome protein plays an important role in actin cytoskeleton dynamics. It weighs about 65 kDa and is widely expressed in diverse tissues but shows higher expression levels in the brain. In its mechanical function N-WASP regulates the polymerization of actin by activating the Arp2/3 complex. Through these interactions it helps in the formation of branched actin networks which are essential for cellular movement and shape.
N-WASP functions are critical in cell signaling endocytosis and neurite extension. N-WASP often acts within a complex and interacts with various proteins like Cdc42 to transmit signals from cell surface to the actin cytoskeleton. These activities position N-WASP as a fundamental component for cellular architecture and mobility impacting processes like immune response and neuronal development. Its interaction with Arp2/3 contributes to dynamic changes in cell movement and shape.
N-WASP plays a role in Rac and Cdc42 signaling pathways important for actin reorganization. Within these pathways it interacts with proteins such as Rho family GTPases influencing cytoskeletal arrangements. The proper functioning of these pathways is vital for numerous cellular processes including the migration of cells which affects tissue development and repair.
Alterations in N-WASP are linked to immunodeficiencies and neurological disorders. Mutations or dysfunction in N-WASP can lead to Wiskott-Aldrich syndrome characterized by immune defects and eczema due to its role in immune cell signaling. Additionally N-WASP's interaction with proteins such as WASP contributes to its involvement in neurodevelopmental disorders like autistic spectrum disorders reflecting the importance of this protein in maintaining normal cellular function.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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