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AB321263

Alexa Fluor® 750 Anti-Nova1 antibody [EPR13847]

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Rabbit Recombinant Monoclonal NOVA1 antibody - conjugated to Alexa Fluor® 750.

View Alternative Names

RNA-binding protein Nova-1, Neuro-oncological ventral antigen 1, Onconeural ventral antigen 1, Paraneoplastic Ri antigen, Ventral neuron-specific protein 1, NOVA1

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13847

Isotype

IgG

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nova1 also known as Neuro-oncological ventral antigen 1 is a member of the Nova family of RNA-binding proteins. It has a molecular mass of approximately 53 kDa. Nova1 is predominantly expressed in the nervous system with high presence in neurons. The protein plays a role in the regulation of alternative splicing by binding to specific RNA sequences. This binding activity influences the splicing of various pre-mRNA transcripts highlighting the role of Nova1 in post-transcriptional gene regulation.
Biological function summary

Nova1 influences neuronal integrity by regulating RNA processing events. It binds to RNA motifs to ensure the accurate splicing needed for correct neuronal function. Nova1 functions as part of a multi-protein complex that includes spliceosomal components which help in recognizing splice sites in precursor RNA. The splicing activity of Nova1 affects the expression of numerous genes related to synaptic function and neurodevelopment which is critical for maintaining proper neuronal communication and plasticity.

Pathways

Nova1 participates in the neuronal alternative splicing pathway and influences synaptic signaling pathways. Its activity affects the pathways by regulating the splicing of various genes critical for these pathways. For example Nova1 is involved with proteins like Nova2 within these pathways. Their combined roles ensure that various proteins essential for synaptic function are produced in the correct isoforms which is necessary for synaptic health and plasticity.

Nova1 has been linked to neurodevelopmental disorders and certain cancers such as brain tumors. It plays a role in these diseases by dysregulating the alternative splicing of transcripts necessary for normal neuronal development and tumor suppression. Nova1 interacts with other proteins such as hnRNP proteins in the context of these disorders which further modulates the disease process. Understanding Nova1's roles helps in developing therapeutic strategies that target aberrant splicing events in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion or exclusion. It binds specifically to the sequences 5'-YCAY-3' and regulates splicing in only a subset of regulated exons (PubMed : 10811881). Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 snRNP binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhances spliceosome assembly and exon inclusion. Binding to 5'-YCAY-3' clusters results in a local and asymmetric action to regulate spliceosome assembly and alternative splicing in neurons. Binding to an exonic 5'-YCAY-3' cluster changed the protein complexes assembled on pre-mRNA, blocking U1 snRNP (small nuclear ribonucleoprotein) binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhanced spliceosome assembly and exon inclusion. With NOVA1, they perform unique biological functions in different brain areas and cell types. Autoregulates its own expression by acting as a splicing repressor. Acts to activate the inclusion of exon E3A in the glycine receptor alpha-2 chain and of exon E9 in gamma-aminobutyric-acid receptor gamma-2 subunit via a distal downstream UCAU-rich intronic splicing enhancer. Acts to regulate a novel glycine receptor alpha-2 chain splice variant (alpha-2N) in developing spinal cord (By similarity).
See full target information NOVA1
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