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AB321651

Alexa Fluor® 750 Anti-Radixin/RDX antibody [EP1862Y]

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Rabbit Recombinant Monoclonal Radixin/RDX antibody - conjugated to Alexa Fluor® 750.

View Alternative Names

Radixin, RDX

  • 578 PE

    PE Anti-Radixin/RDX antibody [EP1862Y]

  • 660 APC

    APC Anti-Radixin/RDX antibody [EP1862Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Radixin/RDX antibody [EP1862Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Radixin/RDX antibody [EP1862Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Radixin/RDX antibody [EP1862Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Radixin/RDX antibody [EP1862Y]

  • Unconjugated

    Anti-Radixin/RDX antibody [EP1862Y]

  • Carrier free

    Anti-Radixin/RDX antibody [EP1862Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1862Y

Isotype

IgG

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Radixin also known as RDX is a member of the ezrin-radixin-moesin (ERM) family of proteins. It weighs approximately 68 kDa and is expressed in a variety of tissues with high levels found in the brain liver and intestines. Radixin functions mechanically by binding to actin filaments and linking them to the plasma membrane. This process is essential for maintaining cell shape and facilitating signal transduction.
Biological function summary

Radixin plays a role in modulating cell surface structure which influences microvilli formation and stability. Radixin serves as a linkage between the actin cytoskeleton and the plasma membrane which helps in forming actin-rich surface structures. It does not function alone; it often acts as part of larger complexes that control cytoskeleton-related activities. Its role in maintaining cellular architecture makes it integral to processes like cell adhesion migration and polarization.

Pathways

Radixin is an important component in signaling pathways that regulate the cytoskeleton and cell membrane interactions. Key pathways include the Rho family of GTPases and the PI3K/Akt pathway. Radixin interacts with these pathways by connecting with proteins such as RhoA which regulates actin dynamics and Akt which influences cell survival and growth. These interactions are important for cellular responses to environmental and internal signals.

Improper function of radixin relates to certain pathological conditions including hepatocellular carcinoma and cholestasis. In hepatocellular carcinoma radixin impacts cell proliferation and migration making it a potential target for cancer therapy. In cholestasis radixin dysfunction has connections with altered bile canalicular structures often interacting with proteins like CFTR. These associations highlight its significance as a potential target for therapeutic strategies aiming to correct cellular structures in these disorders.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
See full target information RDX

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com