Rabbit Recombinant Monoclonal RKIP antibody - conjugated to Alexa Fluor® 750.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor (By similarity).
PBP, PEBP, PEBP1, Phosphatidylethanolamine-binding protein 1, PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP
Rabbit Recombinant Monoclonal RKIP antibody - conjugated to Alexa Fluor® 750.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Raf kinase inhibitory protein (RKIP) also known as phosphatidylethanolamine-binding protein 1 (PEBP1) is a significant regulatory protein weighing around 21 kDa. It inhibits the RAF/MEK/ERK signaling cascade by binding to Raf-1 kinase. RKIP shows expression in diverse tissues including the brain heart and liver reflecting its widespread influence across various physiological systems.
RKIP interacts with multiple signaling pathways by acting as a suppressor of the mitogen-activated protein kinase (MAPK) pathway. It also plays roles in modulating the signaling of nuclear factor kappa B (NF-κB) and G-protein coupled receptor kinase (GRK) pathways. As part of these pathways RKIP contributes to the regulation of cell proliferation differentiation and apoptosis making it an important player in maintaining cellular homeostasis.
RKIP's role centers on the RAF/MEK/ERK and NF-κB signaling pathways. Within the RAF/MEK/ERK pathway RKIP modulates cellular responses by controlling the activation of Raf-1 influencing cell growth and division. In the NF-κB pathway RKIP inhibits phosphorylation of IKK thereby impacting inflammatory responses which links RKIP to proteins like MEK and ERK as well as IKK.
RKIP has involvement in cancer and cardiovascular diseases. Reduced RKIP expression associates with increased metastasis in cancers such as prostate and breast cancer indicating its role as a metastasis suppressor. In cardiovascular contexts RKIP affects hypertension pathogenesis through the modulation of GRK pathway involving connections to proteins such as Raf-1 and IKK.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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