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Rabbit Recombinant Monoclonal SUN2 antibody - conjugated to Alexa Fluor® 750.

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Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Application

Target Binding Affinity

Reactivity

Expected

Dilution info

-

Notes

-

Application

Antibody Labelling

Reactivity

Expected

Dilution info

-

Notes

-

Associated Products

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Target data

Function

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Required for nuclear migration in retinal photoreceptor progenitors implicating association with cytoplasmic dynein-dynactin and kinesin motor complexes, and probably B-type lamins; SUN1 and SUN2 seem to act redundantly. The SUN1/2:KASH5 LINC complex couples telomeres to microtubules during meiosis; SUN1 and SUN2 seem to act at least partial redundantly. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for RAB5-GDP and participate in the activation of RAB5.

Alternative names

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Rabbit Recombinant Monoclonal SUN2 antibody - conjugated to Alexa Fluor® 750.

Alternative names

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR6557

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

Activity summary

SUN2 also known as Sad1 and UNC84 domain containing 2 is a protein that plays a fundamental role in the positioning of the nucleus in cells. It has a mass of approximately 89 kDa. This protein localizes to the inner nuclear membrane where it is involved in tethering the nucleus to the cytoskeleton. SUN2 is mainly expressed in tissues exhibiting high mechanical stress such as muscle and bone tissue. Its structural role anchors the nuclear lamina to the cytoskeleton helping to maintain cellular integrity.

Biological function summary

SUN2 connects the nuclear interior with the cytoskeleton through the LINC complex (Linker of Nucleoskeleton and Cytoskeleton). This complex also includes other proteins like Nesprin and Lamins helping to transmit mechanical signals across the nuclear envelope. SUN2 plays an essential role in processes like cell division nuclear migration and chromosomal positioning. Its function in the LINC complex makes it a significant player in maintaining nuclear morphology and ensuring proper cell division.

Pathways

SUN2 is involved in the mechanotransduction and cell-cycle regulation pathways. In mechanotransduction SUN2 helps translate mechanical signals into biochemical ones therefore influencing gene expression. Related proteins like Nesprin-2 interact within the LINC complex and contribute to these pathways. In cell-cycle regulation the protein helps align the mitotic spindle through connection with components of the cytoskeleton supporting accurate cell division. These pathways demonstrate SUN2's contribution to cellular responses to mechanical stimuli and cell growth regulation.

Associated diseases and disorders

Studies indicate that SUN2 mutations or abnormal expression levels are associated with muscular dystrophy and cancer. In muscular dystrophy defects in SUN2 can lead to impaired nuclear anchoring contributing to muscle weakness. It interacts with Lamin A/C and defects in these proteins are also linked to muscular dystrophies. In cancer SUN2 dysregulation may affect cellular architecture and chromosomal stability contributing to tumorigenesis. Understanding SUN2's roles and interactions offers potential pathways for therapeutic interventions.

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